The Research Of The Relevance Between CD4~+CD25~+FOXP3~+Treg And Graft-versus-Host Disease In Patients After Allo-HSCT

ObjectiveTo observe the percentage of CD4+CD25+FOXP3+Treg in peripheral blood following allo-geneic hematopoietic stem cell transplantation (allo-HSCT) and the relevance between CD4+CD25+FOXP3+Treg and aGVHD, and to primarily explore the possibility of clinical early diagnosis of graft-versus-host disease using CD4+CD25+FOXP3+Treg as a biomarker.MethodsTo detect the ratio of CD4+CD25+FOXP3+Tregin CD4+CD25+T lymphocytes and the expression of FOXP3mRNA in PBMCs of allo-HSCT patients with the flow cytometry and real-time PCR assays.ResultsAfter the transplantation, the ratio of CD4+CD25+FOXP3+Treg in allo-HSCT patients with aGVHD were significantly reduced than the patients without aGVHD and the normal controls (P<0.05), which have the negatively correlated with the severity of aGVHD. Paired analysis in aGVHD patients comparing the ratio of Treg at aGVHD onset to prior time points (7-14days) showed significantly decreased. Expression of FoxP3mRNA were significantly reduced in patients with aGVHD compared with patients who did not develop clinical aGVHD (P<0.01). ConclusionsThe ratio of CD4+CD25+FOXP3+Treg and FOXP3mRNA maybe one of the useful indicators in predicting the occurrence of aGVHD.