Radix Astragali Combined With Insulin Effects On The Levels Of Oxidative Stress In Diabetic Rats

Diabetes is a chronic progressive disease.Currently the incidence ofdiabetes is on the rise,Sustained high blood sugar attack systemicmicrovascular and macrovascular cause of adult blindness, visualimpairment, non-traumatic amputation and kidney failure.DN is themain reason for end-stage renal dysfunction, and there is no effectivedrug to prevent and stop the occurrence of diabetic nephropathy.Thepathogenesis of diabetic nephropathy has not been fully elucidated,multi-factor, multi-mechanism results together, high glucose-mediatedoxidative stress is considered to be one of the important common[3]Theso-called oxidative stress (OS) refers to the increase in reactive oxygenspecies (ROS) or reactive nitrogen clusters produce and/or reducedclearance, resulting in ROS accumulate in the body caused by themolecular, cellular, and damage to the body. Oxidative stress generatedby the activity of free radicals can directly attack the cell membranesurface of unsaturated fatty acids, resulting in lipid peroxidation productmalondialdehyde physiological structure of the cell membrane of thekidney tissue destruction.Under the influence of high-sugar, antioxidantswithin the organization, such as: SOD, GSH-px glycation and oxidation,free radical scavenging capacity of kidney tissue is greatly reduced.TheDM early due to metabolism disorders, and high glucose promoteincreased production of inflammatory cytokine IL-6, and high bloodsugar can lead directly to the mesangial cell damage and the release of IL-6, leading to a vicious cycle.Related studies have reached the level ofIL-6and DM high sugar state, and a positive correlation with theinflammatory condition of the DM[18],Another disorder of glucose metabolism, protein non-enzymatic glycation to generate a largenumber of advanced glycation end products (AGEs), AGEs and itsreceptor interaction, resulting in a large number of ROS.ROS throughactivation of the PKC pathway within the cells of the transcription factorNF-κB activation and start the release of a variety of cytokines, thesecells and inflammatory factors form a complex reaction system topromote the increase of renal tissue.tissue cell collagen synthesisincreased, resulting in hardening of glomerular, start the occurrence anddevelopment of diabetic nephropathy in[19,20,21].Therefore, clarify theresulting oxidative stress mechanism of diabetic nephropathy, looking fordrugs that antioxidants and blockade of oxidative stress, are the keys toprevent diabetic nephropathy.Astragalus has anti-oxidation effect, canreduce the metabolic disorder of free radicals; Astragalus can inhibit theoxidation of the role of glycosylation, can retard the development ofglomerular sclerosis, protect renal function[22].The results of anexperimental study that Astragalus can significantly inhibit brainischemia and reperfusion in rat brain tissue MDA to generate and increasethe activity of SOD to scavenge oxygen free radicals, and fromUltrastructural on confirmed, also must protect its membranousstructure[23].And Astragalus toxicity is small, not easy to produce drugresistance, and has wide application prospect.Thisresearch is mainly by Astragalus antioxidant and scavenging freeradicals, the timely removal of free radicals due to fluctuations in bloodsugar in diabetic rats, In this study, Astragalus antioxidant free radicalscavenging capacity, the timely removal due to blood sugar fluctuationscaused by free radical generation, study the effect of the application ofAstragalus with insulin-treated diabetic rats have lower levels ofoxidative stress and protect the kidneys from oxidative.This experimentprocessing chain urease streptozotocin (STZ) diabetic rat model randomlydivided into normal group, model group, Astragalus group, insulin group and astragalus insulin joint group of five groups, given Astragalus英文缩写英文全称中文全称orally and/or subcutaneous insulin Record blood glucose and bodyweight, measure diabetic rat serum creatinine levels、to detect kidneytissue malondialdehyde (MDA) content, superoxide dismutase (SOD)and glutathione peroxidase (GSH-px) activity, interleukin6(IL-6) andtumor necrosis factor α (TNF-α) content.Malondialdehyde (MDA) is one of the tissue lipid peroxidation endproduct, its content can reflect the organization of cellular lipidperoxidation,TBA chromogenic assay MDA content.The results showedthat compared with the insulin group, the Astragalus Joint insulin groupMDA value significantly lower.The activities of SOD and GSH-pxactivity in the kidney tissue were significantly increased.Interleukin-6(IL-6) and tumor necrosis factor (TNF-α) are importantimmunomodulatory cytokines and inflammatory factors involved in thebody’s inflammatory response.Studies have shown that serum IL-6andTNF-a content as diabetes condition occurs, the detection of thedevelopment and outcome indicators[24].The results show: compared withinsulin group, Astragalus plus insulin group of IL-6, TNF-α valuedecreased significantly. The results of this study show: Astragalus oninsulin treated diabetic rats had reduced levels of oxidative stress andprotect the kidney against oxidative stress injury.