Interconnectivity Between The Reaction System Of Catalase And Hyperlipidemia

Objective: To investigate the interconnectivity between the reaction system ofcatalase and hyperlipidemia.Methods: A total of54unrelated Han Chinese subjects without diabetes （28men and26women） in Northeast China, consisting of2case subjects,hyerlipidemia and coronary mild stenosis subjects （13men and12women, aged62.1±10.07years） and control subjects （15men and14women, aged62.3±10.13years） without hyerlipidemia nor coronary artery disease were enrolled inthis case-control study, which was performed in the Cardiovascular Center, the FirstHospital of Jilin University, Changchun. Patients were diagnosed by coronaryangiography. We measured biomarkers of oxidative stress, such as CAT activity,HNE-LDL, MDA-LDL, Concentration of protein carbonyls, GSH, T-AOC, H₂O₂,and anti-hydroxyl radical antioxidant capacity. Statistical analysis was performedusing the nonparametric Kruskal-Wallis test for comparison of vessel disease andbiomarkers of oxidative stress. Moreover, we analyzed the correlations betweenCAT activity and levels of other biomarkers of oxidative stress by Spearman’scorrelation. Meanwhile, we plotted3-dimensional （3D） figures （independentvariables, CAT activity and H₂O₂levels; dependent variables, levels of otherbiomarkers of oxidative stress）, and analyzed the relation between them by responsesurface regression （RSR）.Results: HNE-LDL level and H₂O₂level of hyerlipidemia group aresignificantly higher than those of the control croup, anti-hydroxyl radicalantioxidant capacity in hyerlipidemia group is significantly lower than that in thecontrol croup （P<0.05）. RSR revealed that in the reaction system of CAT, H₂O₂level significantly contributed to the level of HNE-LDL. In the reaction system ofCAT, the level of H₂O₂significantly contributed to that of HNE-LDL （P<0.05）.However, the reaction system of CAT activity and H₂O₂level did not contribute tothe extent of hyperlipidemia.Conclusions: The oxidative stress levels in patients with hyperlipemia arehigher than that in the persons without hyerlipidemia nor coronary artery disease; the antioxidant capacity in the patients with hyperlipemia is significantly lower thanthat in the the persons without hyerlipidemia nor coronary artery disease. In thereaction system of catalase, the level of H₂O₂significantly contributed to the levelof HNE-LDL. However, there was not the association between the reaction systemof catalase and hyperlipidemia.