Protective Effect Of Hydrogen Saline On Glycerolinduced Acute Renal Failure In Rat Kidney

BackgroundRhabdomyolysis is one of the main reasons that leading to acute kidney injury (acutekideny injury, AKI). The pathogenesis involves multiple physiological factors，Symptomsis differ,the serious can be life threatening.Therefore, early found, early prevention, hasimportant significance for rhabdomyolysis induced acute renal injury. The highintensity mlitary training and strenuous exercise can lead to rhabdomyolysis. How toprevent from rhabdomyolysis early, is an important issue that the present sports medicineand clinical medicine face.PurposeIn this study,the rats model was developed by injecting glycrol into the muscle of ratsto simulate rhabdomyolysis induced AKI.To study hydrogen saline exerts beneficial effecton rhabdomyolysis induced acute renal injury by glycerol (10ml/kg) in rats and toinvestigate the potential mechanism.MethodsWeight of about200g rats were randomly divided into four groups. The control group(Control), model group (Model), large dose hydrogen saline treatment group (Treated1),low dose hydrogen saline group (Treated2). The model group rats were injected50%glyrol (10ml/kg,I. M). Treated1group and treated2were glycerol(10ml/kg, I. M)plus hydrogen saline(10mg/kg, and5mg/kg i.p. route respectively). Used automaticbiohemistry analyzer to measure serum creatine kinase (CK), creatine kinase isozymes(CK-MB), lactate dehydrogenase (LDH), creatinine (Cr), urea nitrogen (BUN).andobserved the renal tissue,to assess the hydrogen salinei’s protective effect onglycerol-induced acute renal failure in rat kidney. Used ELISA to measure reactive oxygenspecies (ROS), glutathione peroxidase (GSH-PX),8-hydmxy-2’-deoxyguanosine (8-OH-dG), interleukin-6(IL-6) and tumor necrosis factor alpha (TNF-a).in thekidney.Used Colorimetric method to measure malondialdede(MDA) and Superoxidedismutase(SOD）.ResultsModel rats, after24hours of injecting glycrol into the muscle, the urine outputsignificant-ly reduced, urine is dark red. Rats kidney morphology inspection, the kidneysize increas-ed and lustre is violet black; By light microscopy,the renal small tube profilewere not clear, renal tubular epithelial cell were necrosis, disintegration and interstitialwere conges-tion, the lumen of the tube type, there are many cell fragments. Serologic testsuggestd that model group serum CK, BUN and CK, CK-MB, LDH were significantlyelevated compared with normal control rats. Model rats, kidney tissue, MDA,8-OH-dG,GSH-PX, IL-6, TNF alpha and ROS levels increased significantly, SOD activity reduced.Treated one group: kidney morphology inspection is obviously improved, serologictesting CK, BUN and CK, CK-MB, LDH reduced significantly compared with modelgroup rats and oxidative stress index that MDA,8-OH-dG, GSH-PX and ROS levels werelower and SOD activity increases, and inflammatory factor IL-6, TNF alpha concentrationdecreased.ConclusionInjecting glycrol into the muscle of rats can simulate rhabdomyolysis inducedAKI,that oxidative stress and inflammatory reaction plays important roles in thepathogenesis of rhabdomyolysis-induced acute renal kidney injury. Hydrogen saline canprotect against renal dysfunction caused by glycerol.The protective effect of hydrogensaline may be involed in its anti-oxidation and anti-inflammatory response.