The Synthetic And Preliminary Applied Research Of Sleep Peptide DSIP

Objective:Analyse purified synthetic DSIP after intranasal absorption and explore its side effects, thus provide a synthetic treatment of insomnia that is not limited by the sources, easy to control the quality, convenient and able to eliminate the undesired side effects so that it can treat insomnia nice and efficiently as exogenous drugs to promote sleeping. Methods:Using the analysis of the presence of bioinformatics of known natural sleep peptide structure choose a isomer and the total chemical synthesis of solid-phase peptide synthesis of DSIP, and by high performance liquid chromatography mass spectrometry analysis and identification of purified DSIP, synthetic peptide that is designed to determine the need for the purpose of peptides and more than95%purity; After grouping animal experiments, rats first preliminary determination whether this synthetic target peptide DSIP role in promoting sleep, to confirm the effect and then a certain dose of the target peptide by intravenous induction as reported in the literature to determine New Zealand’s big ears white rabbits role of sleep, and intranasal administration and to promote sleep effects of different concentrations of DSIP, initially explored the optimal doses of the nasal administration of the purpose of peptide; through Pathology assay of synthetic sleep peptide DSIP in the large and medium-dose and long-term is toxic to brain tissue after administration. Results:1Successful synthesis of biologically active artificial sleep peptide DSIP, by high-performance liquid chromatography after purification the desired purity above95%, mass spectrometry method and HPLC method to confirm the target peptide synthesis is;2Installation of rabbit brain electrode after rabbit still survive in good condition, after the administration of the drug were successfully guide the rabbit EEG, recorded data effectively analysis;3Analysis of EEG and statistically tested, that intranasal administration group, intravenous administration group of rabbits were associated with the physiological saline group in total sleep time, slow wave sleep time differences, and the administration group of slow wave sleep time is long, total sleep time is long;4Analysis of the EEG were statistically tested, Intranasal administration of15nmol/kg and30nmol/kg group and45nmol/kg in total sleep time, slow wave sleep time statistically significant differences, the intranasal administration of30nmol/kg group of rabbits with intravenous dose of30nmol/kg in total sleep time, slow wave sleep there was no significant difference;5Long-term large dose of DSIP after intravenous administration of rat brain slices and the control group by HE no statistical difference. Conclusions:1. Synthetic peptides by intranasal administration of sleep could promote the effectivity of sleep;2. A synthetic peptide of DSIP sleep by intranasal administration of intravenous injection shows similar sleep-improving effect, the preliminary view that the appropriate dose for the nasal administration in rabbits is30nmol/kg;3. There is no significant damage of Synthetic peptide DSIP in long-term sleep and dosage on brain cells.