Study On The Association Between Polymorphism Of CYP19Gene And Gallstone Formation

Objective:This study attempts to set out from the genetic level, through the analysis of related polymorphisms on estrogen metabolism, to investigate the relationship between polymorphism of CYP19gene involved in the formation of estrogen and gallstone formation. It provided a theoretical basis in the early detection and diagnosis of cholecystolithiasis.Methods:Selecting100cases of female cholecystolithiasis patients admitted to the Second People’s Hospital of Chengdu at Hepatobiliary Surgery as a research object during February2010to May2011,100healthy cases as the control group.According to the menopause, all patients were divided into two groups, premenopausal and postmenopausal, there are50cases in each group. The RsaI polymorphism in exon Ⅲ of CYP19gene was detected with the method of polymerase chain reaction-restriction fragment length polymorphism in a polygene case-control study respectively.Through the statistics processing, using the χ2test to compared two groups of genotype frequencies and allele frequency distribution of whether the differences, also using odds ratio on behalf of the relative risk.Results:PCR-RFLP results show that, The frequencies of AA, AG and GG genotypes of CYP19were51%,35%and14%respectively in cholecystolithiasis group, and were30%,50%and20%respectively in control group. Significant differences of frequencies were found in the distribution of Rsal genotypes between the two groups (P<0.05);The frequencies of the two alleles of A and G were68.5%and31.5%in cholecystolithiasis group, and were55%and45%in control group respectively. There was significant difference in the frequency of RsaI alleles between the two groups (P<0.05). compared to AG and GG genotypes, the cholecystolithiasis risk of the female with AA genotype increased by2.429and2.429times respectively. The frequencies of AA, AG and GG genotypes of CYP19were56%,32%and12%respectively in premenopausal group, and were46%,38%and16%respectively in postmenopausal group. There was no significant difference in the frequency of RsaI genotypes between the premenopausal group and postmenopausal group (P>0.05);The frequencies of the two alleles of A and G were72%and28%in premenopausal group, and were65%and35%in postmenopausal group respectively. There was also no significant difference in the frequency of RsaI alleles between the premenopausal group and postmenopausal group(P>0.05).Conclusion:①The polymorphism in exon Ⅲ of CYP19gene may be associated with the incidence of cholecystolithiasis.②The female patients with AA genotype in the Rsal polymorphism in exon Ⅲ of CYP19gene increased the risk of cholecystolithiasis.③Compared to premenopausal and postmenopausal patients,the polymorphism in exon Ⅲof CYP19gene may be not associated with the incidence of cholecystolithiasis.