A Evaluation Of Dynamic Changes Of Mulsle’s Restoration After Damaging Using Acoustic Radiation Force Impulse

ObjectiveThe aim of this study is discuss feasibility and accuracy of the ARFItechnology for evaluate restoration of injuried muscle tissue through explore therelation between Imaging characteristics of the high-frequency ultrasoundandand ARFI, expression of the aFGFand bFGF and pathological changes fromposttraumatic muscles, it can Provide reliablity basis for diagnose of damagedmuscle and therapy of damaged muscle tissue restoration.Methods1, Muscle injury models on20rabbits were establish, then immediatelyobserve using High-frequency Ultrasound and ARFI1day,3day,7day,14dayafter injury. To observe the echo characteristics and internal structure change ofdamaged muscle through High-frequency ultrasound,then measure VTQvalue of damaged muscle using ARFI.2, Injured muscle tissue were taken out, it is fixed for HE light microscopicthrough10%formaldehyde at different observation time, then the pathologicalspecimens were put low and high microscope to observed morphology changeof muscle tissue.3, The muscle issue were measured expression of aFGF, bFGF through theimmunohistochemical method at different time. then analyze relation betweenthe VTQ value and expresstion of aFGF, bFGF after injury.Results1, The nomal muscle fiber tissue were clear, continuity, mostly medium echo. Muscle fiber were partly fractured, insufficient uniformity, slightly strongecho immediately after injury. Damaged muscle tissue were significantly high,mostly hyper echo one days after injury. Muscle tissue were gradually began todecrease, but sightly high echo three days after injury. Muscle tissue weremostly hypoecho seven days, fourteen days after injury.2, ARFI showed the VTQ of muscle issue after injury is higher than muscleissue before injury. The VTQ of muscle issue is the highest7days after injury.The VTQ of muscle issue is began to decrease14days after injury.3, Through Pathological examination, we found that normal musule fiberswere continual and we did not see obvious swelling and bleeding. Fromimmediate to3days after injury, Muscle fiber were rupture, significantly edema,bleeding, hyperemia and inflammatory cell infiltrating.7days after injury, largeareas of muscle cells were necrotic, and amounts of calcium salt depositsed.14days after injury, lots of fiber cells proliferated, and the deposits of calciumobviously reduced.4, The normal muscle tissue can be seen a little expression of bFGFpositive cells, but not can be seen the expression of aFGF positive cells. muscletissue After injury, the expression of bFGF and aFGF is kept rising with theextension of time.The value of VTQ showed negative correlation with theexpresstion of aFGF and bFGF14days after injury. The value of VTQ showednoncorrelation with the expresstion of aFGF and bFGF other groups after injury.ConclusionsAcoustic radiation force impulse imaging results show that the sonogram ofdamaged muscle have their respectivly characteristics different observationtime after injury, and The result of ultrasound elastography were consistent withthese of pathological. ARFI can direct, noninvasivly evaluate the changes ofmuscle tissue restoration after different time of injury, And provide an objectivebasis for the diagnosis and treatment of damaged muscle.