Preparation And In Vitro Performance Evaluation Of Scaffolds Contained With Sericin Protein For Liver Tissue Engineering

All kinds of liver disease have serious impact on human health, the liver tissueengineering technology is the most potential treatment, but looking for the appropriatescaffold materials has been always a technical problems of this field. Silk sericin (SS) is aprecious natural polymer which was used for a long time. The development and utilizationof SS not only can save enviroment, but protect enviroment for it was always discharged asa waster in silk industry. Because of its specific molecular recognition signal, sericin canpromote cell adhesion and proliferation, induce cell differentiation and provide a scaffold。Sericin has received widely attention and studied with its well gel properties, lowimmunogenicity, special biocompatibility and biodegradability, etc. But SS was difficultlycontroled for is is gel in the low temperature and become sol when be heated to roomtemperature, so the modified is indispensable. The current report more aboutthermosensitivity compound gel is Chitosan/Glycerophosphate (CS/GP), is can maintainin sol at room temperature and transform to gel in the range of body temperature, and it canbe a kind of modified material for SS. In addition, Maize Starth (MS) is a kind of completebiodegradable natural polymer which cheap and widely exist. Polyvinyl Alcohol (PVA)with excellent mechanical properties, and good hydrophilia, biodegradability andbiocompatibility, both of them can be used to modify SS as a safety biomedical materials.This topic contain two parts and pretent two kinds of liver tissue engineering scaffoldmaterials which SS/CS/GP thermosensitivity compound gel (injectable in situ hydrogel)and SS/PVA/MS gel film (in vitro formation and regulation), respectively. Analyze thefeasibility of them used in liver tissue engineering scaffolds from their structure, propertiesand application. Part1: SS extracted from abandoned cocoons was mixed with a highdeacelation degree chitosan (CS) and Glycerophosphate (GP) in a special way under icebath. The different proportion of SS/CS/GP complex obtained can maintain sol for a longtime below20℃, and change to gel fast nearby the body temperature environment(37±5℃) and in the range of physical pH value (6.9-7.45). Research of its structure andperformance show that: the SS/CS/GP gel have penetration3-dimensional porousstructure, the proper mechanics strength, good biodegradation and degradation speed canbe regulated, no cell toxicity and have a certain promotion for BRL rat liver cell growthperformance and can be injected in vivo formation. It is expected to be used as a type of insitu liver tissue engineering scaffold.Part2: SS、MS and PVA were prepared to different proportion of the SS/PVA/MS gel membrane in vitro by the water phase blend andrepeated freeze-thaw. The study of its mechanical properties and degradation mechanismshows that: when the mass ratio of SS/PVA was more than3/7and MS add content is18%,the overall mechanical properties of gel film was the optimal. All of the SS/PVA/MScompound gel membrane have degraded property with different ratio, and the dissolvedrate can be regulated by the SS/PVA proportion and the content of MS. This gel membraneis expected to be used in vitro as liver tissue engineering scaffolds with its controlledbiodegradation performance.