Studies On The Chemical Coustituents Of Marine Fungus M1-A4-2and Antarctic Fungus FNJ-4-2

The thesis is divided into three parts: The first part is the thechemicalcoustituents of marine fungus M1-A4-2. The second part is thechemical coustituents of Antarcticfungus FNJ-4-2. The third part is the antibacterial activity of thecompounds.A detailed separation and purification by using thin-layerchromatography (TLC), Sigel column chromatography (CC), gel columnchromatography (Sephadex LH-20), reverse phase columnchromatography (ODS) and high pressure liquid chromatogram-phy(HPLC). Five compounds were isolated from the marine fungus M1-A4-2,and their structures were determined by extensive spectroscopic analysis including1D-NMR (1H-NMR,13C-NMR, DEPT),2D-NMR (1H-1HCOSY,HMBC, HSQC), EIMS and IR. The structures of compounds wereidentified as following:9,10-dihydro-9-one-4,5-Acridinedicarboxylicacid (1＇),(22E,24R)-ergosta-7,22-diene-3β,5a,6β-triol (2＇),4,14,dimethyl-9,19-Cyclocholestan-3-ol-7-one (3＇), Cholesterol (4＇),Bis(2-methylpropyl) ester (5＇).Fourteen compounds were isolated from the antarctic fungus FNJ-4-2,and their structures were identified as following: Ergosterol peroxide (1),cyclo-(Pro-Leu)(2), Ergostan-7-en-3-ol (3),2-(cyanomethyl)-benzoicacid-methyl ester (4),1,2-dihydro-3,5,8-trimethyl-naphthalene (5), cyclo-(Ile-Ala)(6), cyclo-(Pro-Tyr)(7),5,6,7,8-tetrahydro-2-methyl-pyrimido[4,5-b]benzothien-4-ol (8),Ergosterol (9), Uridine(10), Uuracil (11), P-hydroxy-benzaldehyd)(12),Adenosine (13), N1-(2-Furylmethyl)-2-(3-pyridylmethylidene) hydrazine-1-carbothioamide (14).In order to evaluate the antibacterial activity of the compounds, filter paper method was used. Compound1＇and1show the activity inantibacterial, suppressing Bacillus subtilis and Fusarium oxysporumgrowth; compound2＇、3＇ and3also show the activity in antibacterial,suppressing Fusarium oxysporum growth. Other compounds do not showthe activity in antibacterial.