Studies On Growth And Drug Uptake Of Lung Cancer Cells On Nanocomposites By Electrochemistry Impedance Spectroscopy

Biosensors have attracted lots of attention since it has high selectivity, easyautomation, super sensitivity, and rapid response. The evolution of nanomaterials andbiotechnologies offer vast opportunities for the development of biosensor. Biosensorsbased on nanoparticles and sol-gel composites have been used in cell detection, whichprovide an effective way for detection of cancer cells and screening of drugs forinhibiting cancer cell growth.In our experiments, a series of biosensors with nanoparticles and sol-gel basedsurface materials have been developed to realize highly sensitive detection of cancercells, as well as effectively screening of anticancer drugs. The thesis includes thefollowing aspects:(1) One-step electrodeposition of AuNPs and chitosan is implemented tofabricate a nanocomposite substrate for growing HeP-G2cells. The growth of the cellon the nanocomposites surface is characterized by electrochemistry impedancespectroscopy (EIS). The results show that the proposed technique can be used as ahighly sensitive impedance sensor for HeP-G2cells ranging from1×103to1×108cell·mL-1with a linear correlation coefficient of0.9843.(2) MWCNTs-chitosan film is constructed on the glass carbon electrodes byconstant potential electrodeposition. The modified electrode can be used to detect thegrowth of cancer cells and the effect of adriamycin using EIS.(3) AuNPs-MWCNTs-chitosan film is constructed on the glass carbon electrodeby constant potential electrodeposition. The modified electrode is used to detect thegrowth of cancer cells and the effect of adriamycin using EIS. According to thedetection results, the inhibition of cells by adriamycin on the AuNPs-MWCNTs-Chi-tosan film is observed to be stronger than that on the MWCNTs-chitosan film, whichmay be attributed to the doping of AuNPs.