A Study Of Sendai Virus Defective Interfering Particles In Eradicating Neurospongioma

Purpose:Respecting the hypopathogenicity of Sendai Virus for human, meanwhile some Sendai Virus genetypes had been confirmed that had inhibitory action to timor, this thesis will search on the bionomics of Sendai Virus defective interfering particle to tumor, and explore the treatment of tumor via SeV DIP without exogenous gene expression and dissolveing the tumor nor adjunctive therapy. By means of the mechanism of treatment on tumor, a new way will be provide for eradicating cancer.Method:1. Selected the malignant cell strains:A549, MEL526, LiBr, C6, HT29, SW480, A498, the Sendai Virus Tianjin strain was inoculated to these cell strains. Scores of the CPE, cell survival rates, TCID50, PCR of Virus RNA, the sensitive cell strain would be confirmed.2. Preparation of Sendai Virus Tianjin strain defective interfering particle:The Sendai Virus Tianjin strain was obtained by cultivation in the chick embryo. After ultracentrifugation purification, sucrose density gradient purification, checking the multiplication in chick embryo, the SeV DIP would be obtained.3. Inoculated the Sendai Virus Tianjin strain defective interfering particle to the dendritic cell, the cytokins IL-6, TNF-a, IFN-a were detected for evaluating the ability of the Sendai Virus Tianjin strain defective interfering particle to stimulate the dendritic cell in antineoplastic action.4. Made the bearing cancer model. The Sendai Virus Tianjin strain defective interfering particle was injected to the cancer model. Score of the diameter of tumor, cytokine, PCR of tumor RNA, inmuchemistry of tumor, the feasibility of cancer treatment via Sendai Virus Tianjin strain defective interfering particle would be evaluated. Meanwhile the treatment mechanism was initial confirmed.Results:The rat neurogliocytoma cell C6strain was hypesensitive to Sendai Virus Tianjin strain. The TCID50reached to11.24±0.08, and other detecting cell strain could not catch up to this peak (generally from0-3). The experimental animal was confirmed as rat (Wistar). The Sendai Virus Tianjin strain defective interfering particle had ability to stimulating the mutation of dendritic cell. Compared with the control group (inoculated the Sodium Chloride), the cytokines which had the antineoplastic effect (IL-6, TNF-α, IFN-α) ascensus obviously (P<0.001). Compared with the control group (injected the Sodium Chloride), the Sendai Virus Tianjin strain defective interfering particle had ability to stimulating the normal rat to hypeexpress the IL-6, TNF-α, IFN-α (P<0.001). The construction of tumor model was fail.Conclusion:The rat neurogliocytoma cell C6strain was hypesensitive to Sendai Virus Tianjin strain. The Sendai Virus Tianjin strain could cause the CPE and promote the apoptosis obviously and continuely. Sendai Virus Tianjin strain defective interfering particle could promote the maturation of dendritic cell and the antineoplastic material hypoexpressed and conduce to eradicate tumor. Sendai Virus Tianjin strain defective interfering particle could promote rat to hypoexpress the anticancer material. It was benefit to the neurogliocytoma treatment. Because of the fail cancer model, the in vivo activity of Sendai Virus Tianjin strain defective interfering particle had not been demonstration.