Kinesin Family Member6Trp719Arg Variants And Coronary Artery Disease:a SNP Association Study

Objectives:Kinesin family member6(KIF6) protein widely exists in eukaryotic species, expressed in many tissues and cell types, including skin, connective tissue, brain and vascular systems. It is involved in the intrancellular transport along microtubles; the cargos transported include membrane organelles, protein complexes, and mRNAs. KIF6Trp719Arg polymorphism (20455C/T) has been found to be the risk to Caucasus with coronary heart disease (CHD) by linkage analysis and association analysis of the genomewide. To date, no genetic-epidemiological data about coronary artery disease (CHD) are available for Chinese Han nationality. The aim of this study was to explore KIF6Trp719Arg polymorphism distribyting and the characteristic, also went to explore the relationship between KIF6Trp719Arg polymorphism and CHD.Methods:All candidates were inpatients who came from vasculocardiology deparment in the Afflliated Hospital of Medical College of Armed Police Forces between the March2008and August2009.307patients were divided into CHD group and control group. The CHD group comprises239patients diagnosed as CHD, ranging from37to85years old (63.7±10.6y),143of whom were male.The numbers of major epicardial coronary culprit arteries(left main artery, left anterior descending, circumflex, and right) with=1significant stenosis (=50%) were recorded.68patients without CHD in the same period, ranging from42to78years old (59.9±9.9y),23of whom were men, were set as control group. Patients with CHD were further classified into MI and non-MI subgroup by combining past history with a thorough review of medical records on diagnostic electrocardiogram and enzyme changes. We also chose sex as an independent factor to analysis. We compiled basic information including sex, age, body mass index and so on,and blood samples were drawn for test.20455C/T was genotyped by TaqMan alle polymorphism analysis for all the samples. Quantitative data were assessed using the Student’s t-test. Associations between qualitative variables were analysed with the χ2test or Fisher exact test, when indicated. Multivariate logistic regression model was used to exclude the influence of confounding factors and determine the interaction between polymorphisms and the risk of CHD or MI. Statistical analyses were performed with the software package SPSS version17.0.Results:1. Some variables of patients’ clinical characteristics in case group and control group are separately displayed in table2. The percentages of men,smoking and a history of diabete are higher than the control group (P<0.05).The mean age of the patients in the CHD group is also higher. Systolic pressure, fasting plasma levels of glucose, total cholesterol, low density lipoprotein, high density lipoprotein, triglycerides and the rate of hypertension, CHD family history, hyperlipidemia, ischemic shoke,didn’t have a significant difference between the two groups.(P>0.05).2. The genetype frequency of20455C/T TT、CT、CC are31.4%,47.6%,21.0%, in the Han nationality of tianjin, China.3. In univariate analysis, KIF6Trp719Arg genetype was no significantly different between case and control group (P>0.05). Multivariate analysis also indicated no relationship between KIF6Trp719Arg and CHD.4. There is no link between KIF6Trp719Arg and atherosclerosis of coronary arteries. Through futher analysis, subgroups were divided according to the phenotype including Myocardial infarction (MI) and non-Myocardial infarction (non-MI). Univariate analysis showed KIF6Trp719Arg was no significantly different. So did Multivariate analysis. In univariate analysis, men or women group showed KIF6Trp719Arg was no significantly different. But multivariate analysis showed there was may be some association between KIF6Trp719Arg polymorphism and MI in men and women.Conclusion:The present investigation in the Han nationality provides no evidence that the KIF6gene Trp719Arg might be relavent with susceptibility or severity to CHD.