Effects Of Hydrogen Molecules On Adipose Tissue Lipid Metabolism And Lipid Metabolism-related Gene Expressions In Chow-diet-fed C57BL/6Mouse

Objective:The purpose of the present study was to demonstrate the effects of hydrogen molecules on lipid metabolism and its mechanisms via studying the lipid content and lipid metabolism-related gene expression, as well as the phosphorylation of AMPK and its downstream protein ACC in adipose tissue in chow diet C57BL/6mice.Methods:All6-week-old C57BL/6mice in chow diet were randomly divided into two groups and one group was given intraperitoneal injection of hydrogen saturated saline and another was given normal saline for4weeks.Concentrations of total cholesterol (TC) and triglyceride (TG) in plasma and adipose tissue were determined by enzymatic methods.HE staining was used to observed the morphological changes of adipose tissue cells.Lipid metabolism-related genes expression in adipose tissues,such as leptin, adiponectin and hormone-sensitive lipase and others were measured by quantitative real-time PCR.The expressions of phosphorylated AMPK and ACC protein and its phosphorylated protein were detected by Western blot analysis.Results:1.Compared with the control group,plasma TC and TG of hydrogen-saturated normal saline group decreased significantly by18.9%and33.4%, respectively.2.Compared with the control group, the morphological changes of adipose tissue in the hydrogen-saturated normal saline group were that the cell outline was clearer, the number of cells in the same field of vision was more, the diameter was shorter and the cell section area was smaller.3. Concentrations of TC and TG in adipose tissue were lower in hydrogen-saturated normal saline than that of the control group by43.9%and20.1%, respectively.4.Hydrogen-saturated normal saline increased significantly the gene expression of adiponectin hormone, hormone-sensitive lipase (HSL), sterol regulatory element binding transcription factor1(SREBTF-1), peroxisome proliferator-activated receptor (PPARy), and reduced the mRNA expression ofleptin,TNF-a,IL-6.Although the lipoprotein lipase gene expression was increased, but the up lever was not obvious (P>0.05).5.Hydrogen-saturated normal saline increased the phosphorylation levels of adipose tissue adenylate activated protein kinase (AMPK) and its downstream protein Acetyl coenzyme A carboxylase (ACC) by29.4%and61.3%, respectively, compared with the control group.Conclusion:1.Hydrogen molecules not only reduces the plasma TC, TG content, but also decreases the deposition of lipid in the adipose tissue cells.2.Hydrogen molecules are able to up-regulate adiponecti、hormone-sensitive lipase SREBF1, PPARy and LPLmRNA expression and down-regulate leptin, TNF-a and IL-6gene expression, thereby playing an important role in regulating lipid metabolism in adipose tissue.3.Hydrogen molecules can activate AMPK and thus inhibit its downstream protein ACC activity in adipose tissue which may be one of the molecular mechanisms by which hydrogen molecules regulate the lipid metabolism of adipose tissue.