A Preliminary Study On Relation Of Hypermethylation Of Secreted Frizzled-related Protein (SFRP2) Gene In Peripheral Blood With Colorectal Cancer

Objective To investigate the promoter hypermethylation of secreted frizzled-related protein2(SFRP2) gene in peripheral blood of colorectal cancer, and to discuss the potential of secreted frizzled-related protein2gene hypermethylation as a reliable biomarker in occurrence and development of colorectal cancer.Subjects SFRP2gene hypermethylation in peripheral blood of colorectal cancer Materials and Methods1) collection of preoperative peripheral blood samples from colorectal cancer patients, then placed them in-20℃low temperature refrigerator.2) Methylation-specific PCR was used to detect DNA methylation of the SFRP2gene in peripheral blood samples.3) Statistical analysis:the use of SPSS17.0software for statistical analysis.Results Hypermethylated SFRP2was found in60.8%(42/69),56.2(27/48),4%(2/50) of blood samples from rectal carcinoma, colon carcinoma, and benign intestinal disease,respectively. In contrast, no hypermethylated SFRP2was detected in blood of normal controls. Moreover, it is found that SFRP2methylation has positive relation with tumor stage, differentiation and lymph node metastasis. But no significant associations were observed between hypermethylated SFRP2and clinicopathological features including sex, age, tumor stage and location.Conclusion Hypermethylation of SFRP2gene in peripheral blood has some associations with CRC, and might be a high potential biomarker for the early screening of CRC.