Effects Of Qiweibaizhusan On On The Intestinal Microorganisms And Enzyme Activity

Objective:In order to construct dysbacteriotic mice model of diarrhea and understand the curative effect of ultra-micro Qiweibaizhusan.To study the effects of Jianwei Qiweibaizhusan, Sijunzitang and Qiweibaizhusan on intestinal microorganisms and enzyme activities, and to confirm the scientific property of prescription compatibility on QiweibaizhusanMethods:The dysbacteriotic mice models were constructed by using traditional Chinese medicine compound and combination antibiotics to cause diarrhea, and then treated with ultra-micro powder of Qiweibaizhusan.Microbial culturing methods and enzymological methods were used to determine the number of microbes and enzyme activities in gastrointestinal tract.70Kuming mice were randomly divided into7groups:the control group, the antibiotics treated group,the minus agastache group, the minus costustoot group, the minus kudzuvine root group, Sijunzitang group andQiweibaizhusan group. The control group was treated with boiled water, the antibiotics group was treated with antibiotics mixture composed of gentamycin sulfate and cefradine, all other groups were treated with antibiotics mixture and Chinese medicines.Results:Traditional Chinese medicine dachengqitang had quick effect on diarrhea. The symptom of insufficiency of the spleen would appeared in mice with long time traditional Chinese medicine dachengqitang treatment. But it had low effect on intestinal microbes(P>0.05). The combining using of gentamycin sulfate and cefradine had the best effect on diarrhea.After treated with ultra-micro powder Qiweibaizhusan, traditional Chinese medicine dachengqitang group were cured quickly and the symptom of insufficiency of the spleen disappeared.The quantities of intestinal lactobacillus and fungus in antibiotic group were much higher than the normal group (P<0.01).After three days of treatment with Chinese medicine, the intestinal microbes and enzyme activities of intestinal contents were measured. The results showed that the color of mice feces turned from black to yellow, the diarrhea mice treated by the minus kudzuvine root group and Qiweibaizhusan group were cured. Compared to the antibiotics group, the amount of bacteria from the minus kudzuvine root group and Qiweibaizhusan group showed significant reduction (P<0.05).Compared to the control group, the amounts of Bifidobacterium spp. and Lactobacillus spp. from each treated group significantly increased (P<0.05).Compared to the control group the amounts of bacteria, Bifidobacterium spp. And Lactobacillus spp. from the antibiotics treated group, significantly reduced (P<0.05), but the amount of fungi was significantly increased (P<0.05).Compared to the antibiotics group, the amount of fungi from each Chinese medicine treated group significantly increased (P<0.05). Compared to the control group, intestinal enzyme activity from every treated group significantly reduced (P<0.05). Compared to the antibiotics treated model group, protease and amylase activities from each every Chinese medicine treated group were significantly higher (P<0.05). Xylanase activities of the minus agastache group and Qiweibaizhusan group were significantly lower than that of antibiotics treated group, the minus agastache group and Sijunzitang group (P<0.05).Conclusion:A dysbacteriotic mice modeling method of diarrhea was constructed. There were some kinds of ingredients contained in ultra-micro powder Qiweibaizhusan which could promote the growth of intestinal lactobacillus and fungus.The ingredients of tonifying qi which exist mainly in Sijunzitang, Ginseng, Rhizoma Atractylodis and Poria cocos, are the key Chinese medicine for the regulation of intestinal physiological flora.Agastache and costustoot played important roles on anti-diarrhea by inhibiting the growth of bacteria and the xylanase activity, and kudzuvine root had the function on improving the amylase activity.