Effects Of Estrogen Receptor And Exercise-induced Stress On Testosterone-induced CPP In Mice

Testosterone is one of the anabolic androgenic steroids （AAS） derivatives, the mostcommon drug in athletic events. In recent years, a series of animal experiments and clinicalstudies showed that the AAS has a reward and reinforcement effect which would producephysical and psychological dependence just as the classical drugs. However, compared withthe classical opioid drug abuse, the study of the mechanism of AAS abuse is not clear. Itwonders whether the exercise-induced stress affects the susceptibility of body andpsychological dependence.Objective:This article used behavioral pharmacology methods, mainly studies on estrogen receptorin central nervous system and exercise-induced stress on testosterone-induced conditionedplace preference in mice （CPP） roles. This article has certain significance in expounding AASand AAS on the central role of the reward system behavioral pharmacology of abusemechanism.Methods:The role of Estrogen receptor to the reward effect on testosterone used conditioned placepreference test. First in order to determine testosterone formation in mice CPP best dose of0.5mg·kg^-1,1mg·kg^-1,2mg·kg^-1mice were injected subcutaneously. Each2d is a trainingcycle. Later, before training CPP gives estrogen receptor antagonist ICI182,780or Tamoxifen（sc） forms testosterone CPP to conduct the effect test to the mice.To the movement stress training test experiment, mice were given testosterone （sc） afterhigh-intensity treadmill training.30min to carry on the CPP training, Method was same to thefront. Results：1) The effects of training for the expression of testosterone CPP in mice.Testosterone trained mouse CPP12d,0.5mg·kg^-1，1mg·kg^-1，2mg·kg^-1group CPP scoresclearly is higher when compare with the control vehicle group （P <0.05）.2) The effect of sc estrogen receptor antagonist ICI182,780for the expression oftestosterone CPP in mice.SC ICI182,780could significantly weaken the CPP scores of the expression oftestosterone formation in mice （P <0.05） but when sc ICI182,780alone could not formationCPP in mice.3) The effect of sc estrogen receptor antagonist Tamoxifen for the expression oftestosterone CPP in mice.SC Tamoxifen can significantly weaken the CPP scores of the expression of testosteroneformation in mice （P <0.05） but when sc Tamoxifen alone could not formation CPP in mice.4) The effect of exercise-induced stress on expression of testosterone CPP in miceexercise-induced stress mice and non exercise-induced stress mice group CPP scores clearly ishigher when compare with the control vehicle group （P <0.05）.But the exercise-induced stressmice and non exercise-induced stress mice group CPP score did not differ significantly aftertraining （P>0.05）.Conclusion:Different doses of testosterone can be formation of CPP in mice. Estrogen receptorantagonist ICI182,780and Tomoxifen could significantly inhibit the expression oftestosterone CPP. Exercise-induced stress did not significantly improve the expression oftestosterone of the CPP in mice.The result indicate that testosterone has reward property inmice, and estrogen receptor may play a role in the reward of testosterone. Exercise-inducedstress method used in this article may not directly work on rewarding effects of testosterone.