The Proliferation And Apoptosis Effects Of Metformin On Ovarian Cancer Cell SKOV3in Vitro

Purpose:To study the effect that metformin has on the proliferation in vitro andapoptosis of human ovarian cancer cells, to observe whether the combination ofmetformin and pacltaxel has a synergistic reaction on SKOV3cells.Method:1.Cell Cuture:Rescitiating ovarian cancer SKOV3cells byconventional method,vaccinating cells in RPMI-1640culture medium containing10%fetal bovine serum (FBS).at37℃,5%CO2saturated humidity incubator.2.CellProliferation Inhabition Eperiment (MTT reduction method): Culturing SKOV3cellsin cuture medium containing0,0.01,0.5,1,5,10mmol/L concentration of metformin,respectively by MTT reduction method, detecting inhibition of cells proliferation ineach group in the first24,48,72hours.3.Flow Cytometry (FCM): Culturing SKOV3cells for48hours in culture medium containing0,1,5,10mmol/L concentration ofmetformin for48hours. With FCM, making a quantitative analysis of the change ofcell cycle and apotosis rate in each group.4.With0.5,1.0mmol/L concentration ofmetformin and600ug/L of1200ug/L paclitaxel., sigle or jointly culturing cells todetect the proliferation of cells in each group in the first48hours.Result:1.Metformin Inhibits the Proliferation of SKOV3Cells: Adopting MTTreduction method to detect cell proliferation inhabition rate in each group.,depictingthe growth curve,suggesting the slow growth of SKOV3cells in cultutre mediumcontaining different metformin concentration,reflecting significant growth inhibitionand concentration-and-time dependence,while cells of the control group growwell.2.The Results of Flow Cyometry;The results of flow cyometry show that, after48hours in different concentration of metformin, compared with the control group,with the increase of the concentration of metformin,the apoptosis rate of experimentalgroup SKOV3cells was significantly higher(P<0,05),the cell cycle changedsignificantly, with the increase of metformin concentration,the proporation of cells atG0/G1phase decreased,while metformin inhibits the proliferation of SKOV3cells atG2/M phase.3.The combination of metformin and paclitaxel has a synergistic reaction on SKOV3cells,with gradual increase of the concentration,the inhibition ofproliferatio gradually increased, which has a statistical significance.Conclusion:1.Metfor,im can inhabit the proliferation of ovarian cancer cellsSKOV3,inducing apoptosis of cells.2.Metformin can block the proliferation of ovarian cancer cells SKOV3atG2/GM phase.3.The combination of metformin and paclaxel has a synergistic reaction on theproliferation of ovarian cancer cells,and metformin may increase the sensitivity of thechemtherapy drugs.