Effects Of Mesenchymal Stem Cells From Mouse Bone Marrow On Phenotype Of Allogeneic Spleen Derived Dendritic Cells

Objective：To investigate the effects of mesenchymal stem cells from mouse bonemarrow on phenotype of spleen derived dendritic cells and the mechanism of action.Methods：C57BL/6mice bone marrows cells(BMSCs) were isolated in vitro and thenco-clutured with spleen derived dendritic cells of C57BL/6murine at the same ratio forfive days.According to the processing methods, the experiment is divided into two groups,namely control group (A) and experimental group (B).Group A:dendritic cells+lipopolysaccharide (LPS=1ug/ml); Group B:dendritic cells+BMSCs+LPS (1ug/ml),BMSCs:DC=1:1. The morphological of DC were observed by scanning electronmicroscopy and the phenotypes of DC in the two groups were examined by flowcytometry.Results:1. Compared with A group,the expression of ILT4and CD45RB of DCincreased obviously in B group（P<0.05）;The expression of CD11c,MHC Ⅱand CD86decreased distinctly（P<0.05）;The expression of PDL-1,CD40,CD45RA,CD1d and CD14were no significant difference（P>0.05）.2. The number of synaptic of DC in A group ismore than the DC which were untreated with LPS, however, The number of synaptic ofDC in B group is less than the DC which were untreated with LPS.Conclusion: BMSCs can enhance the expression of ILT4and CD45RB and abate theexpression of CD11c, MHC2and CD86; BMSCs can reduce the synaptic of DC and the toinhibit antigen presenting capacities of DC so as to induce the immunological tolerance.