Anti-inflammatory Effects Of Orientin-2"-O-β-D-galactopyranoside In Lipopolysaccharide Stimulated Microglia

Object: To investigate the anti-inflammatory and neuroprotective effects of2"-O-beta-D-galactopyranosylorientin in LPS-stimulated microglia cells.Method: The inflammation states was manifested by the inflammatory cytokinesand enzymes, such as NO, TNF-α, iNOS,COX-2, and IL-1β. The level of nitric oxide(NO) in the Supernatant was examined with Griess assay and tumor necrosis factor(TNF-α) was tested with ELISA. The mRNA levels of iNOS,COX-2,TNF-α, IL-1β inthe LPS-stimulated microglia cells were examined by Real-time PCR assay. And, thelevels of iNOS and COX-2protein in the stimulated microglia cells were assayed bywestern blot. Then, NF-κB translocation was analyzed by immunofluorescence, IκB-αand ERK1/2protein expression by western blot. Therefore, the neuroprotective effect ofOGA was detceted by the in vitro glia/neruon co-culture system.Results: All of three flavonoids extracted from Trollius chinensis Bunge caninhibit the stimulation of microglias. And, OGA showed the most pontent activity. OGAcan inhibit the increase of iNOS,COX-2,TNF-α, IL-1β induced by LPS. Besides, OGAcan suppress the degradation of IκB-α, reduce the nuclear translocation of transcriptionfactor NF-κB, and subsequently inhibit the phosphorylation of ERK1/2. Moreover,OGA can protect neuron cells from the inflammatory injury in the.microglia/neuronalco-culture system.Conclusion: OGA inhibited LPS-induced microglial activation by blocking theIκB/NF-κB signal pathway and the ERK1/2phosphorylation.