The Study On The Effects Of Trans Fatty Acid On Neurobehavioral In Mice

Objective: To establish the neurobehavior test combinations of micemovement and learning-memory. Using these combinations to investigate theinfluence trans fatty acids exert on mice neurobehavior, which would providethe scientific basis for risk assessments of trans fatty acids.Methods: After adapting7days, the mice were divided into five groupsrandomly,12males and12females in each. The animals in group1,2,3,4were given TFA in feed (0.25%，0.5%，1%，2%) and control group were givenordinary feed. The consumptions of feed were recorded every3days and theweights of mouse were recorded every7days. After12weeks, theneurobehavioral tests were determined by the test combination called Tailsuspension test (TST), forced swimming test (FST), open field test (OFT) andMorris water maze. The data were analyzed by IBM SPSS Statistics, One-wayANOVA and repeated measures ANOVA.Results: All the mouse did not died in the whole experiment. Comparedwith control group, there were no significantly changes in weight duringbreeding (P>0.05). There was no significantly difference in time staying in thecenter area in each group in OFT (P>0.05). In FST experiment the time of M5group was obviously longer than that of M4, there were significantlydifferences between these two groups (P<0.05), while there were nosignificantly differences between other groups (P>0.05). There were nosignificantly differences in all the groups of immobility time in TST (P>0.05).The difference of escape latency was no statistically significant in Morriswater maze (P>0.05).Conclusion: According to the results of neurobehavioral tests, It wasconfirmed that the TFA of low measurement（less than2％）would not changethe neurobehavior of the mice. In this study, trans fatty acids on mice neurobehavior was explored for the first of time and the experimental dosewas arranged according to the content of TFA in Chinese meal. It also neededto proceed for long-term, high-dose experimental studies.