Study On The Quasispecies With Disease Relationship Of Hepatitis B Virus With In S Area Gene In Acute On Chronic Liver Failure

Objective:In this topic, the relation between distribution characteristics of S area quasispecies of HBV genes and liver disease development is explored through testing S area quasispecies stripe numbers of HBV genes in the patients of CHB group and the patients of acute on chronic liver failure caused by hepatitis B virus.Methods:select20cases of patients with acute-on-chronic liver failure caused by HBV and20cases of patients with CHB (control group). Take limosis vein blood from the patients and carry out IMX test and extract HBV-DNA serum and test the carrying capacity of HBV-DNA. Test the HBV genetic typing, conduct2%agarose gel electrophoresis analysis (2%concentration) to the products that are obtained from HBV-DNA serum through nested PCR amplification. Carry out SSCP analysis on all serum specimens so as to observe the quasispecies conditions of HBV-DNA in the patients’serum. Use SPSS17.0software package to conduct the statistical analysis on the data.Results:1. There is not any statistical difference (P>0.05) among sex, age, genotype and HBeAg status of two groups of the patients and there is statistical meaning (P<0.05) in differences among HBV-DNA carrying capacity, TBIL, PT, PTA%.2. There is statistical meaning (P<0.05) in comparative difference among quasispecies stripes of the patients with CHB and Patients with liver failure. The complexity of S area Quasispecies of HBV-DNA genes in the serum of the patients with liver failure is more complicated than that of the patient with CHB.3.There is not any statistical meaning (P>0.05) in the comparative differences of PCR-SSCP stripe numbers of the patients with chronic hepatitis B whose HBV genotypes are B and C. The patients with liver failure whose genotype is B have more stripe numbers than that of patients whose genotype is C so there is the statistical meaning (P<0.05) in such comparative differences. In general, quasispecies stripe numbers of the patients with genotype B are more than that of the infected persons with genotype C.4. The HBeAg negative quasispecies stripe numbers of the patients with CHB and liver failure have more stripe numbers than that of the patients with HBeAg positive so there is the statistical meaning (P<0.05) in such comparative differences.5. Carry out correlation analysis on the quasispecies stripe numbers of the patients with CHB and liver failure and their ALT and HBV-DNA carrying capacity and find that there is not any correlation between ALT and HBV-DNA carrying capacity so there is not any statistical meaning (P>0.05)Conclusions:1. It is confirmed that S area quasispecies of HBV genes in the patients with CHB who have not received antiviral therapy and the patients with acute on chronic liver failure caused by HBV do exist.2. The stripe numbers of SSCP quasispecies of patients with CHB are less than that of the patients with acute on chronic liver failure so there is the statistical meaning in the differences.3. There is not any statistical meaning in the differences in the quasispecies stripe numbers in the patients with CBH whose genotypes is B and C types. In the patients with liver failure, the quasispecies stripe numbers of genotype B are more than that of genotype C. In general, In general, quasispecies stripe numbers of the patients with genotype B are more than that of the infected persons with genotype C.4. In the patients with CBH and liver failure, quasispecies stripe numbers of the patients with HBeAg negative are greater than that of the patients with HBeAg positive.5. There is not any linear dependence between carrying capacity of ALT and HBV-DNA and quasispecies stripe numbers, which can show the increase of quasispecies heterogeneity is related to the host, virus itself, immunologic mechanism and external environment.