The Role And Mechanism Of PTP1B In Gastric Bypass Surgery To Treating Type2Diabetic Rats

Objective: To explore the function and mechanism of Roux-en-Y gastricbypass in treatingt of T2DM, we research the treatment effect of RYGB inT2DM rats, explore the relationship of protein tyrosine phosphatase1B withT2DM, investigate the role and possible mechanism of PTP1B in gastricbypass surgical treatment of T2DM rats.Methods:(1)8-weeks-age male SD rats80, were randomly divided intohigh-fat diet group (n=40), normal food diet group (n=40), respectively, to behigh fat diet and normal diet8weeks, and the rats fasting weight wasmeasured weekly fixed point in time.(2) After fed high-fat diet8weeks, givenstreptozotocin (STZ)(25mg/kg) by intraperitoneal injection to establishingthe model of T2DM rats.(3) The T2DM rats were randomly divided intoRYGB group (DRO, n=18), sham-operated group (DSO, n=10); normal fooddiet group were randomly divided into RYGB group (NRO, n=12),sham-operated group (NSO, n=12).(4)The fasting body weight of rats in eachgroup were determined in preoperative,4weeks and8weeks after surgery.(5)The FBG、FINS、TG、TC and HOMA-IR of rats in each group weredetermined in preoperative,4weeks and8weeks after surgery.(6)The rats ineach group were sacrificed to acquiring the Liver and skeletal muscle in8thweek after surgery, and apply Immunohistochemical staining and WesternBlot semi-quantitative determined the distribution and expression level ofPTP1B in Liver and skeletal muscle. Results:1The comparison of weight between high-fat group with normal dietgroup in8weeksAt the beginning of the modeling, the comparison of weight between twogroups had no statistically significant (P>0.05), the comparison of weightbetween two groups had statistically significant in4th weeks and8th weeks(P <0.05).2The comparison of weight in four groups in preoperative andpostoperativeIn the preoperative, the weight of DRO group and DSO group comparedwith the NRO group and NSO group, and had statistically significant (P <0.05); the comparison of weight between DRO group and DSO group had nostatistically significant (P>0.05); the comparison of weight between NROgroup and NSO group had no statistically significant (P>0.05). In thepostoperative, the weight of DRO group that in4th weeks and8th weekscompared with preoperative had statistically significant (P <0.017); theweight of NRO group that in4th weeks compared with preoperative had nostatistically significant (P>0.017), the weight in8th weeks compared withpreoperative had statistically significant (P <0.017); the weight of the othertwo groups that in4th weeks and8th weeks compared with preoperative hadstatistically significant (P <0.017).3The comparison of FBG in four groups in preoperative andpostoperativeIn the preoperative, the FBG of DRO group and DSO group comparedwith the NRO group and NSO group, and had statistically significant (P <0.05); the comparison of FBG between DRO group and DSO group had nostatistically significant (P>0.05); the comparison of FBG between NRO group and NSO group had no statistically significant (P>0.05). In thepostoperative, the FBG of DRO that in4th weeks and8th weeks comparedwith preoperative had statistically significant (P <0.017); the FBG of theother three groups that in4th weeks and8th weeks compared withpreoperative had no statistically significant (P>0.017).4The comparison of FINS in four groups in preoperative andpostoperativeIn the preoperative, the FINS of DRO group and DSO group comparedwith the NRO group and NSO group, and had statistically significant (P <0.05); the comparison of FINS between DRO group and DSO group had nostatistically significant (P>0.05); the comparison of FINS between NROgroup and NSO group had no statistically significant (P>0.05). In thepostoperative, the FINS of DRO that in4th weeks and8th weekscompared with preoperative had statistically significant (P <0.017); the FINSof the other three groups that in4th weeks and8th weeks compared withpreoperative had no statistically significant (P>0.017).5The comparison of HOMA-IR in four groups in preoperative andpostop-erativeIn the preoperative, the HOMA-IR of DRO group and DSO groupcompared with the NRO group and NSO group, and had statisticallysignificant (P <0.05); the comparison of HOMA-IR between DRO group andDSO group had no statistically significant (P>0.05); the comparison ofHOMA-IR between NRO group and NSO group had no statisticallysignificant (P>0.05). In the postoperative, the HOMA-IR of DRO that in4thweeks and8th weeks compared with preoperative had statistically significant(P <0.017); the HOMA-IR of other the three groups that in4th weeks and8th weeks compared with preoperative had no statistically significant (P>0.017).6The comparison of TG in four groups in preoperative and postoperativeIn the preoperative, the TG of DRO group and DSO group comparedwith the NRO group and NSO group, and had statistically significant (P <0.05); the comparison of TG between DRO group and DSO group had nostatistically significant (P>0.05); the comparison of TG between NRO groupand NSO group had no statistically significant (P>0.05). In the postoperative,the TG of DRO that in4th weeks and8th weeks with preoperative hadstatistically significant (P <0.017); the TG of DSO that in4th weeks and8thweeks compared with preoperative had statistically significant (P <0.017);the TG of the other two groups that in4th weeks and8th weeks comparedwith preoperative had no statistically significant (P>0.017).7The comparison of TC in four groups in preoperative and postoperativeIn the preoperative, the TC of DRO group and DSO group comparedwith the NRO group and NSO group, and had statistically significant (P <0.05); the comparison of TC between DRO group and DSO group had nostatistically significant (P>0.05); the comparison of TC between NRO groupand NSO group had no statistically significant (P>0.05). In the postoperative,the TC of DRO that in4th weeks and8th weeks compared with preoperativehad statistically significant (P <0.017); the TC of DSO group that in4thweeks compared with preoperative had no statistically significant (P>0.017);the TC of DSO group that in8th weeks compared with preoperative hadstatistically significant (P <0.017); the TC of the other two groups that in4thweeks and8th weeks compared with preoperative had no statisticallysignificant (P>0.017).8In the postoperative, the results of WB and Immunohistochemical for PTP1B expression in liverThe PTP1B of DSO group compared with the other three groups, andhad statistically significant (P <0.05); The PTP1B of DRO group comparedwith NSO group and NRO group, and had statistically significant (P <0.05);the comparison of PTP1B between NRO group and NSO group had nostatistically significant (P>0.05).9In the postoperative, the results of WB and Immunohistochemical forPTP1B expression in skeletal muscleThe PTP1B of DSO group compared with the other three groups, andhad statistically significant (P <0.05); The PTP1B of DRO group comparedwith NSO group and NRO group, and had statistically significant (P <0.05);the comparison of PTP1B between NRO group and NSO group had nostatistically significant (P>0.05).Conclusion:1In the postoperative the weight and FBG of T2DM rats have reduced,improved the insulin resistance and corrected the Metabolic disorders ofLipids, proved that RYGB have a exactly treatment for T2DM.2The expression levels of PTP1B in liver and skeletal muscle of T2DMrats was higher than normal rats, suggested that PTP1B related to theoccurrence and development of Metabolic disorders of Lipids, obesity andT2DM, was expected to become a new target for treatment of T2DM.3In the postoperative, The expression levels of PTP1B have reduced inthe liver and skeletal muscle of T2DM rats, may be one of the mechanismsfor RYGB to treatment T2DM.