Expression And Significance Of Hedgehog Signaling Pathway And E-cadherin In MNNG Induced GES-1

Background and PurposeCell adhesion molecule is a Glycoprotein which Exist The surface of the cellmembrane to Mediated Cell and matrix, Cell-to-cell’s Mutual adhesion. Among,E-cadherin Is an important signal transduction molecules and cell adhesion molecules,Involved in tissue morphogenesis, cell fate and embryonic development, Its mainfunction is to control cells mobile. Adhesion molecule closely related to the loss orabnormality often associated with tumor invasion and metastasis. E-Cad-depth studywill help to reveal the mechanism of the occurrence and development of malignanttumors.Hedgehog(Hh) Signaling pathway is Important signaling molecules in animalembryogenesis, Regulate cells, Abnormal activation of the Hh signaling pathway wasfound in a growing number of tumor, Plays an important role in the signaling pathwaymay be transformed in the stem cells into cancer stem cells, The signal peptide Hhsignaling was highly expressed in a variety of tumors and cancer cell lines, Has beendemonstrated in a variety of tumor cells, Gastric cancer is harmful to human health ahigh incidence and high mortality diseases. Factors for gastric cancer mainly due tophysical and chemical factors, chemical factors accounted for in our country. Nowmore and more chemicals are found to have carcinogenic effects in cell and animalexperiments, One of the most commonly used is MNNG, MNNG synthetic nitrosocompounds, can cause serious damage to cellular DNA, resulting in a variety oflesions induced cell cancer, The study showed that MNNG can cause human gastricepithelial cells (GES-1) a series of malignant transformation, we established an invitro model of malignant transformation, facilitate the study of the incidence ofgastric cancer, the development process and its mechanisms.The test is primarily toexplore MNNG GES-1after E-Cad expression of the Hh pathway and its significanceand impact on the ability of MC proliferation. Method1, The MNNG role GES-1cells and induce their malignant.2, MTT assay toobserve GES-1cells group MC cells with the ring target Ming interference expressionof the growth cycle of the cell group.3Semi-quantitative RT-PCR observed threegroups of cells Shh, Gli1gene expression.4, The fluorescence confocal observe threegroups of cells in the expression of E-Cad.ResultMTT colorimetric experimental growth curve show the MC group> ring targetMing interference group> GES-1cell group, The difference was statisticallysignificant, the the fluorescence immunoassay display ring target Ming interfere withthe group, the average fluorescence intensity of213±35, less than GES-1cells group825±134, greater than MC cell group57±12(p <0.05);semi-quantitative RT-PCRshowed ring target-ming interference group Gli1mRNA of value of0.3241±0.0023, significantly lower than the group’s MC cells.5792±0.0134(p <0.05),ConclusionHh blocker ring target Ming role of MC cells after, Gli1mRNA reducedexpression of E-cadherin expression was elevated, suggesting that the Hh signalingpathway involved in the regulation of E-cadherin expression may Gli1. And enhancedthe MC cell proliferative activity in vitro.