| Objective CD4~+CD25~+Foxp3~+Treg, Th17and myeloid-derived suppressor cells(MDSCs) are considered to be the most critical cells participating in tumor immune escapemechanism. This studies were detected the changes of Treg/Th17, MDSCs expressions inthe peripheral blood of patients with gastric cancer, to preliminary approach to the role ofCD4~+CD25~+Foxp3~+Treg, Th17, MDSCs in the development and progression of the gastriccancer.Methods The peripheral blood specimens from77cases gastric cancer,21cases gastricintraepithelial neoplasia,33cases atrophic gastritis,45cases gastric ulcer and20caseshealthy controls were collected. The CD4~+CD25~+Foxp3~+Treg expressions were measuredby flow cytometry, and the CD4+IL-17+Th17expressions after the co-stimulation of PMAand Ionomycin were also measured by flow cytometry The correlations between the Tregand Th17expressions with age, sex, tumor location, TNM stage, depth of invasion andlymph node metastasis of gastric cancer were preliminary analyzed based on the clinicaldata. The peripheral blood mononuclear MDSCs percentage were measure by flowcytometry, and the correlation between the MDSCs with age, sex, tumor location, TNMstage, depth of invasion and lymph node metastasis of gastric cancer were preliminaryanalyzed.Results (1) The peripheral blood percentage CD4~+CD25~+Foxp3~+Treg cells of CD4+T cell in gastric cancer[（4.72±1.01）%] was significantly higher than in gastricintraepithelial neoplasia[(3.23±0.38)%], in atrophic gastritis[(2.57±0.41)%], in gastriculcer [(2.02±0.63)%], in healthy controls[（1.57±0.99）%](p<0.01), and were showedstatistically significantly difference among the five groups(p<0.01). The peripheral bloodTh17percentage of CD4~+T cells in gastric cancer[（8.16±3.13）%]was significantlyhigher than in gastric intraepithelial neoplasia[(6.80±2.12)%], in atrophic gastritis[(5.79±1.40)%], in gastric ulcer[(4.94±1.06)%] and in healthycontrols[(4.85±1.85)%], and were showed statistically significantly difference among thefive groups(p<0.01). The peripheral blood percentage CD4~+CD25~+Foxp3~+Treg cells ofCD4~+T cell in gastric cancer patients were correlated to the depth of infiltration,lymphatic metastasis, and clinical TNM stages, but were no related to the age, gender andtumor location. The peripheral blood Th17percentage of CD4~+T cells in gastric cancerpatients were no related with the age, gender, clinical TNM stages, depth of infiltration,and lymphatic metastasis.(2) The peripheral blood mononuclear MDSCs percentage in gastriccancer[（21.72±10.12）%] were significantly higher than in gastric intraepithelialneoplasia[(13.16±3.79)%], in atrophic gastritis[(7.74±1.14)%], in gastric ulcer[(4.79±1.07)%], in healthy controls[（2.90±1.80）%], and were showed statisticallysignificantly difference among the five groups(p<0.01). The peripheral blood mononuclearMDSCs percentage in advanced gastric cancer （IIA, IIB, IIIA, IIIB, IIIC,IVstage)[（23.79±9.48）%] was significantly higher than in early gastric cancer（IA, IBstage）[（11.74±4.01）%](P<0.05), but were no related with the age, gender, depth ofinfiltration, and lymphatic metastasis in gastric cancer patients.(3)The peripheral blood mononuclear MDSCs percentage were showed positiverelation to the peripheral blood percentage CD4~+CD25~+Foxp3~+Treg cells of CD4+Tcell(r=0.680) and the peripheral blood Th17percentage of CD4~+T cells(r=0.724).Conclusion (1) Gastric cancer patients may exist the high Treg and Th17cellsexpressions in peripheral blood, That suggested there is a disturbance of Treg/Th17ingastric cancer.(2) The peripheral blood mononuclear MDSCs percentage in gastric cancerpatients is remarkable rise, which may be related with low immune function of gastriccancer and development in the gastric cancer... |
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