The Immunomodulatory Effects Of Fuqizhihan Granule And Yupingfeng San On Immunosuppressive Mice

Objective:To discuss the influence of Yupingfengsan and Fuqizhihan granule on inherent immunity and adaptive immunity in mice, compare the difference of Yupingfengsan and Fuqizhihan granule in improving mice immunity, and provide some experimental basis for clinical application.Methods:Kunming mice (60male and60female) were randomly divided into8groups:normal group, model group, Yupingfengsan high dose group, Yupingfengsan middle dose group, Yupingfengsan low dose group,Fuqizhihankeli high dose group,Fuqizhihankeli middle dose group,Fuqizhihankeli low dose group.Make immunosuppression model mice through introperitoneal inject cyclophosphamide except normal group for seven days. gavage normal saline to normal group and model group, Yupingfengsan to Yupingfengsan high, middle, low dose group and Fuqizhihan granule to Fuqizhihan granule high, middle, low dose group once a day respectively for14days. determine the weight of thymus and spleen precisely and calculate the immune organ index; detect the phagocytosis of monocyte-macrophages through neutral red method, proliferation of T lymphocyte by MTT assay, killing activity of natural killer cells by LDH-release method, contents of CD3+, CD4+, CD8+, CD19+on flow cytometry, contents of serum hemolysin by HC50assay, serum contents of IL-1, IL-4and IFN-y by ELISA.Results:1, Every dose group in Yupingfengsan and Fuqizhihan granule can improve innate immunity of Immunosuppressed mice in different degree. Compared with saline group, all indexes in cyclophosphamide group were statistically significant difference, besides, low dose group could improve killing activity of natural killer cells, phagocytosis index, serum IL-1β,in certain extent, but compared with saline group had statistically significant difference (P<0.05). Compared with cyclophosphamide group, all index except killing activity of natural killer cells and IL-1β had statistically difference.2, The influence of two compounds on adaptive immunity. CD19+,IL-4serum hemolysin, IFN-y, proliferation of T lymphocyte and contents of CD3+, CD4+, CD8+in cyclophosphamide group had statistically significant difference compared with saline group (P<0.05). low dose group could improve CD19+,IL-4serum hemolysin, IFN-y in some extent, but compared with saline group had statistically significant difference(P<0.05). Besides, compared with cyclophosphamide group low dose group could not improve CD19+,IL-4serum hemolysin, IFN-y (P>0.05), but high, middle dose group could improve adaptive immunity in some extent (P<0.05)Conlusion:Both Yupingfengsan and Fuqizhihan granule could improve immunosuppressive mice immunity, the result revealed that their effect was similar. Both two compounds were superior in restoring cell immunity for immunosuppressive mice. Middle dose group had the best effect.