Expression And Significance Of DLX3and Syncytin In Placenta Tissue Of Preeclampsia

Research background and objectivePreeclampsia is a common disease in hypertensive disorders in pregnancy, which is a systemic disease and affects the maternal and infant health, leading to multiple organ function damage and even collapse, therefore it is the main reason of maternal and perinatal death. In recent years, more and more attention has been paid on the placenta trophoblastic hypoxia ischemia and shallow placental implantation theory, domestic and international experts and scholars of preeclampsia have done much in-depth research about it, to explore the factors which effect the placental trophoblast cells growth and differentiation has become one of the hot spots for the study of the pathogenesis of preeclampsia.DLX3gene is a a member of homeobox gene DLX family, which contains a highly conserved sequence of transcription, plays an important role in many aspects of embryonic growth, the new study shows that DLX3gene may be involved in placental trophoblastic cells growth and differentiation, their relationship between preeclampsia has not been reported. Syncytin is a sugar envelope protein coded by human endogenous retrovirus defective virus of W (HERV-W), Mi first reported Syncytin can influence placental cytotrophoblast differentiation. This study detected the expression of DLX3and Syncytin in placenta of preeclampsia to explore the possible role and relationship of the pathogenesis of preeclampsia between DLX3and Syncytin.This paper chose preeclampsia patients as the experimental group, selected normal pregnant women as control group, Color doppler ultrasound detected fetal umbilical artery blood systolic/diastolic ratio and biparietal diameter in the mild, severe preeclampsia and the control groups within there days before the termination of pregnancy. Also the DLX3mRNA, Syncytin mRNA and protein were detected by semi-quantitative RT-PCR and enzyme-linked immunosorbent assay(ELISA). The relationship between DLX3and Syncytin was determined.Materials and MethodsClinical medical information60women with preeclampsia(study group)(including30cases of mild preeclampsia and30cases of severe preeclampsia) and30normal pregnant women(control group) were recruited from the patients at termination of pregnancy in the First Affiliated Hospital of Zhengzhou University between2011March and2011December, diagnostic standard of preeclampsia was referenced to the seventh edition of the textbook of Obstetrics and Gynecology by Jie Le. The average age and deliver weeks of mild preeclampsia were31.69±3.61years old and35.81±2.16weeks, while the severe preeclampsia patients were32.16±4.82years old and34.73±2.92weeks, the normal pregnant women are28.62±2.81years old and37.91±2.51weeks. the research objects had no other pregnancy complications, and each group at age, maternal time, etc, was matched, the difference was not statistically significant (P>0.05).Experimental methodsSampling the placenta after delivery, the DLX3mRNA and Syncytin mRNA and protein were detected by semi-quantitative RT-PCR and enzyme-linked immunosorbent assay(ELISA). The relationship between DLX3and Syncytin was determined.Statistical analysisSPSS17was used for statistical analysis, data is represented by x±s, the data were treated by one way ANOVA, comparison among groups was by LSD method, correlation analysis was by Pearson correlation, P<0.05means significant difference. Experimental results1. The level of mRNA expression in placenta tissue of preeclampsia:RT-PCR quantitative analysis indicate that the mRNA relative expression of DLX3, Syncytin in mild and severe preeclampsia were both lower than the control group. The severe were lower than the mild, and the differences are significant(P<0.05).2. The protein expression of DLX3, Syncytin in placenta tissue of preeclampsia: ELISA quantitative analysis indicate that the protein expression of DLX3, Syncytin in mild and severe preeclampsia were both lower than the control group. The severe were lower than the mild, and the differences were significant(P<0.05).3. The correlation analysis of DLX3and Syncytin indicate that the mRNA expression of DLX3and Syncytin in mild and severe preeclampsia were positively related(r=0.429, P<0.05; r=0.513, P<0.05), the protein expression of DLX3, Syncytin in mild and severe preeclampsia were positively related(r=0.579, P<0.05; r=0.677, P<0.05).4. The correlation of expression of DLX3and Syncytin with umbilical artery blood systolic/diastolic ratio and biparietal diameter, the protein expression of DLX3and Syncytin were negatively to umbilical artery blood systolic/diastolic ratio(r=-0.624, P<0.05; r=0.637, P<0.05), were positively related to biparietal diameter(r=0.536, P<0.05; r=0.617, P<0.05).Conclusion1. The mRNA and protein expression of DLX3, Syncytin were decreased in the placenta in preeclampsia, suggesting that they may relate to the pathogenesis and severity of preeclampsia.2. DLX3mRNA and protein have a positive relationship with Syncytin, so we infer that DLX3may be one of the upstream regulatory factors of Syncytin, both of them participate in the guidance of cytotrophoblast fusion and differentiation towards syncytiotrophoblast cells, and causing preeclampsia.3. The protein expression of DLX3and Syncytin have a negative relationship with of patients with preeclampsia, and have a positive relationship with biparietal diameter of fetus.