Studies On Chemical Constituents Of Lysimahcia Christinae Hance

Jinqiancao, the dry whole herb of Lysimachia christinae Hance, which belongs to family Primulaceae, exhibits activities of heat-clearing and detoxifying, activating blood and dissolving stasis, and diuretic and eliminating calculi. It has long been used as an effective treatment for biliary calculus, hepatolithiasis, bladder caluli, and kidney stone. In this paper, the research progress in recent years was firstly reviwed on the chemical constituents and pharmacological activities of genus Lysimachia, especially focusing on the progress of studies on L. christinae. Then, the chemical constituents was investigated on the title plant.The specimen material of L. christinae was collected in Beibei, Chongqing. After being dried, the plant was ground and percolated with95%C2H5OH at r.t. The ethanol extracts was successively extracted with petroleum ether, EtOAc, and n-BuOH to yield several parts with different polarities. The EtOAc-soluble part was subjected to repeated column chromatography on SiO2, polyamide, Sephadex LH-20, and macroporous resin, as well as semi-prep. HPLC, followed by recrystallization, respectively, to give11compounds. These separation methods were also used on the n-BuOH-soluble part to yield3compounds. The structures of the14compounds were elucidated on the basis of the spectroscopic analyses, including UV, HR-ESI-MS, and ID-,2D-NMR.Among the14compounds isolated from L. christinae, twelve are flavonoids, and two are phenolic acids. They were identified, respectively, as myricetin3,3’-di-O-a-L-rhamnopyranoside (1), quercetin3,3’-di-O-a-L-rhamnopyranoside (2), quercetin3-0-a-L-rhamnopyranosyl-(1â†'2)-β-D-galactopyranoside (3), quercetin3-O-rutinoside (also called rutin)(4), kaempferol3-O-a-L-rhamnopyranosyl-(1â†'6)-β-D-glucopyranoside (5), kaempferol3-O-a-L-rhamno-pyranosyl-(1â†'2)-β-D-glucopyranoside (6), kaempferol3-O-a-L-rhamnopyranoside (7), kaempferol3-O-β-D-galactopyranoside (also called trifolin)(8), quercetin3-O-β-D-galactopyranoside (also called hyperin)(9), quercetin3-O-β-D-glucopyranoside (also called isoquercitrin)(10), myricetin3-O-a-L-rhamnopyranoside (11), amentoflavone (12),3,4-dimethoxybenzoic acid (13), and3,4,5-tri-hydroxybenzoic acid (also called gallic acid)(14). Compounds1and2were new flavonoids. Compound12was known but reported for the first time from the genus Lysimachia and3,13,14were known but isolated from this plant for the first time.