Investigation On Hepatitis A Virus Infection In Swines

Objectives:1. To detect the hepatitis A virus in live attenuated hepatitis a vaccine（H2strains）withRT-PCR. Then, same way is used to detect the hepatitis A virus in blood and bilesamples of human.2. To test the serum prevalence of swine hepatitis A virus infection with serologicaltechnology.3. To detect the hepatitis A virus infection in blood and bile samples of swine,discussing the feasibility of that swine is used for animal models of hepatitis Ainfection.Methods:1. To detect the hepatitisAvirus in live attenuated hepatitis a vaccine with RT-PCR.2. In total,27blood samples and79bile samples has been gathered to detect thehepatitisAvirus infection.3. In total,181swines blood was collected in two tubes, one was used to get serum bycentrifuging, the serum was tested for HAV antibodies; the other was used to detectthe hepatitisAvirus infection.4.136bile samples of swines have been gathered to detect the hepatitis A virusinfection with RT-PCR.Results:1. Successfully clone target gene of the hepatitis A virus in live attenuated hepatitis avaccine with RT-PCR.2. The positive rate of HAV is7.41%and1.17%respectively in27blood samples and79bile samples. 3. There are3models of HAV antibodies: model1(HAV IgM+，HAV IgG-), model2(HAV IgM-，HAV IgG+), model3(HAV IgM-，HAV IgG-),and their rates are6.48%，58.01%，35.91%.4. No target gene has been cloned from7blood samples and136bile samples ofswines with RT-PCR.Conclusions:1. Successfully build the RT-PCR to detect hepatitis A virus in live attenuated hepatitisa vaccine.2. The positive clone rate of blood samples and79bile samples HAV infection is7.41%and1.17%respectively.3. There is higher antibody positive rate of HAV in swine, and the infection model issimilar to human infection model.4. No target gene has been cloned from samples of swines with RT-PCR. Whetherswines can be infected with HAV of human can not be confirmed.