| Objective To investigate the relationship between postoperative cognitive dysfunction (POCD) of aging model rats and hippocampus inflammatory response. Furthermore, to observe the therapeutic effect of electroacupuncture on rats suffered from POCD through regulate inflammatory response.Methods Ninety male SD rats (1month old) were randomly divided into adult group (group A, n=15) and aging model group(n=75), the second one was divided into elder group (group E, n=15), postoperative3days group (group P3d, n=15), postoperative7days group (group P7d, n=15), electroacupuncture3days group (group EA3d, n=15) and electroacupuncture7days group (group EA7d, n=15). Rats in group A received intraperitoneal injection of0.9%saline (60mg/kg/d, total42days) to compare with other groups. Rats in the other five groups received intraperitoneal injection of5%D-galactose (60mg/kg/d, total42days) to prepare for making aging model. Group P and group EA were adopted the model of partial hepatectomy. Group P3d got Y-maze for detecting cognitive function after24hours of postoperation, total3days, and group EA3d received electroacupuncture and Y-maze for detecting cognitive function after24hours of postoperation, total3days. Group P7d got Y-maze for detecting cognitive function after5days of postoperation, total3days, and group EA7d received electroacupuncture and Y-maze for detecting cognitive function after5days of postoperation, total3days. The other three groups directly got Y-maze after24hours of intervening measures, total3days. Experiment indicators:(1) Detected cognitive function by Y-maze;(2) Measured the levels of IL-1β, IL-6and TNF-a in blood serum by enzyme linked immunoadsorbent assay (ELISA);(3) Detected CD200, CD200R, activated microglial and astrocyte expression in hippocampus by immunohistochemistry.Results There was no significant difference in weight, cognitive function and expression of IL-1β, IL-6, TNF-β, CD200, CD200R, activated microglial and astrocyte between group A and group E (P＞0.05). Compared with group A, Group E rats’hair color is dimmer, motion is slower. Compared with group E, Group P3d rats’hair color is dimmer, motion is slower, the number of times of running to the safe arm decreased significantly (P＜0.01) and the average time-consuming of running to the safe arm prolonged significantly (P＜0.01) in group P3d. But there was no significant difference in cognitive function between group P7d and group E (P＞0.05). Compared with group P3d, the number of times of running to the safe arm increased significantly (P＜0.01) and the average time-consuming of running to the safe arm shortened significantly (P＜0.05) in group EA3d. But there was no significant difference in cognitive function between group EA7d and group P7d (P＞0.05). Compared with group E, the levels of IL-1β, IL-6increased significantly (P＜0.01) and the level of TNF-α increased non-significantly (P＞0.05) in group P3d, however, the levels of IL-1β, IL-6and TNF-α increased non-significantly (P＞0.05) in group P7d. Compared with their corresponding group P, the levels of IL-1β, IL-6increased significantly (P＜0.05) and the level of TNF-α increased non-significantly (P＞0.05) in group EA3d, but all above three indicators changed non-significantly (P＞0.05) in group EA7d. Compared with group E, activated microglial and astrocyte expression in hippocampus pituitary cell increased significantly (P＜0.01) and CD200decreased significantly (P＜0.01) and CD200R decreased non-significantly (P＞0.05) in group P3d. Compared with group P3d, activated microglial and astrocyte expression in hippocampus pituitary cell decreased significantly (P＜0.01) and CD200increased significantly (P＜0.01) and CD200R increased non-significantly (P＞0.05) in group EA3d. Compared with group E, all of these four indicators changed non-significantly (P＞0.05) in group P7d. Compare with group P7d, all of these four indicators changed non-significantly (P＞0.05) in group EA7d.Conclusion (1) After surgery, the proinflammatory cytokines in peripheral blood can induce hippocampus inflammatory response by direct and indirect ways, and this phenomenon may relate to postoperative cognitive dysfunction of aging model rats.(2) After surgery, activated microglial and astrocyte expression in hippocampus pituitary cell increased and CD200decreased, and this result would promote hippocampus inflammatory response.(3) Decline of aging model rats’ cognitive function may improved by electroacupuncture therapy, because electroacupuncture can inhibit hippocampus inflammatory response by decrease the expression of proinflammatory cytokines in peripheral blood.(4) Decline of aging model rats’ cognitive function may improved by electroacupuncture therapy, because electroacupuncture can inhibit the hippocampus microglia and astrocyte activation and improve the ability of CD200to regulate microglia activation. |
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