The Study On The Relationship Between Ischemia Modified Albumin With Severe Preeclampsia And Early Damage Of Neurological System In Newborns

Objective：Determination on the content of ischemia-modified albumin（IMA） in pregnant women with severe preeclampsia and normalpregnant women, IMA and S100b protein in neonatal cord blood, observationon differences of peripheral blood IMA between the patients with severepreeclampsia and the normal pregnant women, relationship between levels ofS100b protein and IMA in cord blood of neonates, study on correlationbetween the severe preeclampsia pregnancy and neurological systemdamage on early neonatal, making IMA become a biomarkers, as aprediction of severe preeclampsia, in the purpose of providing reliableobjective basis for early diagnosis, thereby reducing infantile cerebral palsy,mental retardation and other sequelae.Methods：60patients who had prenatal examinations and delivered in theObsterics of the Second Hospital of HeBei Medical University were selectedduring October2011to June2012. Among which,20cases are with earlyonset of severe preeclampsia,20cases with the late onset severe preeclampsia,and20cases of normal pregnant women as control group. The same groupingwas conducted for the newborns. Severe preeclampsia diagnosis standard canbe referred in the seventh version of <Obstetrics and Gynecology>.According to the diagnosis standard, severe preeclampsia before34w isdefined as early onset, and that after34w were defined as late onset.3ml ofanconeal median venous blood were collected from the subjects in the study,and afterwards the blood sample (aider separating plasma)was immediatelypreserved for assaying the plasma level of IMA, HSA and S100b protein．Results：1Plasma levels of serum IMA/HSA in pregnant women.: Plasma levels of serum IMA/HSA in pregnant women with early onset of severepreeclampsia is0.5456±0.08, Plasma levels of serum IMA/HSA in pregnantwomen with late onset severe preeclampsia grop is0.2814±0.02, Plasmalevels of serum IMA/HSA in pregnant women of The control group is0.0623±0.01. Plasma levels of serum IMA/HSA were elevated in women withpreeclampsia compared to women with normal pregnancy.(P<0.01).And it inthe early onset severe preeclampsia was also higher than it in late onset severepreeclampsia (P<0.01)．2IMA levels in cord blood: IMA levels in cord blood with early onsetof severe preeclampsia is13.26±1.18, IMA levels in cord blood with lateonset of severe preeclampsia is8.36±1.06, IMA levels in cord blood withnormal pregnancy is3.15±0.81. IMA levels in cord blood with severepreeclampsia is significantly higher than it in cord blood with normalpregnancy (P<0.01).And it in the early onset severe preeclampsia was alsohigher than it in late onset severe preeclampsia (P<0.01)．3S100b protein levels in cord blood: S100b protein levels in cord bloodwith early onset of severe preeclampsia is1437.97±132.3, it in cord bloodwith late onset of severe preeclampsia is958.05±115.5, and it in cord bloodwith normal pregnancy is449.45±84.88. S100b protein levels in cord bloodwith severe preeclampsia is significantly higher than it in cord blood withnormal pregnancy(P<0.01).And it in the early onset severe preeclampsiawas also higher than it in late onset severe preeclampsia (P<0.01)．4.IMA andS100b protein levels in cord blood with severe preeclampsia had no significantpositive correlation (r=0, P <0.01).Conclusion: first, peripheral blood serum IMA/HSA of Severepreeclampsia in pregnant women was significantly higher than those in normalpregnant women, in which severe preeclampsia with early onset ones is higherthan the that in the ones with late onset. It shows that maternal peripheralblood serum IMA may become a new biomarkers in predicting severepreeclampsia. Secondly, the content of IMA, S100b protein in serum of cordblood with severe preeclampsia was significantly higher compared to that in normal newborns, which confirmed the damage on the brain andneurological system of neonatal due to hypertension in pregnancy. It alsoshows that the level of IMA in serum of cord blood can be used as a biologicalmarker. Thirdly, there was no significant correlation between IMA and S100bprotein in cord blood of pregnant women with severe preeclampsia. It showsthat the IMA level in neonatal cord blood could only be served as apredication of early brain and neurological system, but not the degree ofdamage.