Hydroxypropyl - Beta - Cyclodextrin Complex Magnetic Particle Synthesis Characterization And Application In Targeted Drug Delivery

HP-β-CD is widely used pharmaceutical excipients approved by most regions and countries of the Food and Drug Administration due to its properties of stability to light, base and acid, improved complexing ability, greater water solubility, and lower toxicity. Recently, the HP-β-CD was introduce to the surface of magnetic nanoparticles and have attracted much attention because of is prospective application as a drug carrier in magnetic drug targeting systems. However, the reported synthesis method of HP-β-CD composite magnetic nanoparticles (HPCDMNPs) was chemical coprecipitation, which resulted in wide distribution of particle size and not stable in aqueous solution. So it is still a challenge to synthesize the monodispersed HPCDMNPs. Herein, the monodispersed HPCDMNPs were synthesized by pyrolysis of oleic acid iron complex and then was transfer to water phase. Finally the HP-β-CD was coupled on the magnetic particles surface by condense reaction. Besides the determination of carboxyl group on the surface of MNPs was also studied. The detailed results were as follows:1:Synthesis method of HP-β-CD composite magnetic nanoparticles:The HPCDMNPs was prepared by a three-step method of high-temperature decomposition of iron-oleate complex, aqueous phase transfer, and condense reaction. Then the obtained HPCDMNPs was characterized by TEM、FT-IR、TGA and DLS. The experimental results showed that the particle size of HP-β-CD composite magnetic nanoparticles are12-14nm. The HP-β-CD content is17.8%.2:Cycotoxicity evaluation and drug-loading and release study on HPCDMNPs:The cycotoxicity evaluation of HPCDMNPs was investage by using the MTT methods with HepG2cell line. And the antitumor drug of Doxrubicin (DOX) was used to study the drug-loading and release properties. The results showed that at the concentration of600mg/ml. the survival rate of cell was still higher than70%. which indicated the the HPCDMNPs have good biocompatibility. The drug content of Dox is14%with90%loading effecientcy, and Cumulative drug release showed the sustained release of Dox in viro.3:The method to determine of carboxyl group onthe surface of magnetic nanoparticles was established by coupling4-(bromomethyl)-6,7-dimethoxy coumarin (BMMC) with carboxyl group. The reaction condition, such as reaction time and reactant ratio was optimized. Compared to the titration of conductivity, the established method has the advantages of wider detection range, higher sensitivity and stability with less sample amounts.