Shell - Karyotype Of Multifunctional Nanoparticles Synthesis, Characterization And Application Of Biomedical Research

Gene therapy has shown great potentials in the treatment of a number of humandiseases, for instance, genetic diseases, cancer. However, there are still some bigchallenges. Development of efficient and safe gene delivery systems is meaningful forits potential applications. Due to the limitations associated with viral vectors, includingnon-targeting, low loading ability and unsafety, more attentions have been paid todevelop non-viral vectors.In this study, core-shell nanoparticles of Fe₃O₄@SiO2were prepared using reversemicromulsions method. These fabricated nanoparticles show excellent biocompatible，water-soluble, dispersibility properties. The sizes of Fe₃O₄@SiO2nanoparticles arecontrolled within a mean diameter of approximate50nm, and with a zeta potential of-39.1mV. After surface modification by poly（allylamine hydrochloride）（PAH）, the zatapotential of Fe₃O₄@SiO2/PAH nanoparticles was changed to+41mV. The modifiednanoparticles still shows good superparamagnetism. The Fe₃O₄@SiO2/PAHnanoparticles （NPs） could staticeletrotronically attract EGFP-N1plasmid tosuccessfully mediate gene transfer in cultured HeLa cells. Our data showed that theNPs-DNA complexes prevented DNA degradation in a dose-and time-dependentmanner in the presence of serum. Moreover, we have found that NPs-DNA complexeswould be complete uptake into HeLa cells within6h. This novel maganetic targetednano-carrier has perspective applications.VEGF plays an important role in vasculogenesis and angiogenesis， our studyshows that NPs complex can efficiently mediate targeted VEGF plasmid transfer inendothelial cells。 The transfection efficiency is related to the weight ration ofFe₃O₄@SiO2and PAH. The efficiency even reached90%when the ratio is up to100.Targeted VEGF plasmid is able to successfully express in endothelial cells andsignificantly promote cell proliferation. The Fe₃O₄@SiO2/PAH&pVEGF complexoffered a new therapy approach for wound healing and cardiovascular disease, it showshuge applications in gene delivery.