The Synthesis And Application Of Boric Acid Compounds

Phenylboronic acid compounds have attacted more attention just because theirunique physical and chemical properties.Currently, the detection methods based on theunique property of gold nanoparticles(AuNPs) has been extensively applied in protein、DNA、metallic ion and studying the affection between biological molecules. In thispaper, we report a functionalisation assay based on AuNPs for detecting lectin protein.The carboxy group of ithiothymine dipropionate and the amino group of3-amino-phenylboronic acid sulfate directly to happen dehydration by adding1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride(EDC) to form a kind ofmercapto phenylboronic acid derivative(Bor), and the product was characterized byFourier-transform infrared spectroscopy(FT-IR) and matrix-assisted laser desorptionionization time-of flight mass spectrometry(MALDI-TOF-MS).A bifunctional strategy, which enable conjugate phenylboronic acid andpentapeptide CALNN as stable ligand to gold nanoparticles. By using bornic acidscould recognize cis-diol configuration in saccharides in aqueous media, the3’-Sialyllactose(SL) was firstly motified on the gold nanoparticles, which can beemployed to detect three lectins Canavalia ensiformis Agglutinin(ConA)、SambucusNigra Agglutinin(SNA) and Maackia Amurensis LectinⅡ(MALⅡ), and analyze theconjugates by UV-visible spectroscopic and transmission electronic microscopic (TEM)techniques. The assay could be used as a optical probes for monitoring specfic affinitiesof lectins with3’-Sialyllactose.