| Epidural adhesion formation is believed to be a central governing factor in the prevalence of pain after spinal surgery and is regarded as being the primary instigator of neural tethering, while the application of anti-adhesion material is a kind of effective method to prevent it. Sodium hyaluronate which is an ideal anti-adhesion has a positive effect on inhibit bleeding and anti-inflammatory, and a certain concentration of it can inhibit fibroblast proliferation in laminectomy site. Preliminary studies have demonstrated that Sodium hyaluronate (SH) is effective in preventing early postlaminectomy epidural adhesion formation, because degradation of the gel in vivo is faster. Therefore, in this study, polysaccharide was choosed to fabricate blend film incorporate with Sodium hyaluronate, in order to slow down the degradation rate of the blend films for preventing long-term epidural adhesion formation.1. Above all, the sodium hyaluronate/Konjac glucomannan (KGM) blend films were fabricated via making the sodium hyaluronate and Konjac glucomannan as materials, making Lactic acid as an additive to decrease the solution viscosity with microwave treatment and adopting the hybrid solution of Ammonia and anhydrous alcohol as cross-linker. The influencing factors including the content of SH, KGM and Lactic acid, and treatment time of microwave and crosslink were analyzed via the orthogonal experiment L16(4’5).The results show that solution viscosity were around90～6267mPa·s, thickness was between41.8～54.5μm, tensile strength and elongation of the blend film reached about11.3～20.7MPa and10.8～20.2% respectively, and water absoption rate reached151～508%, while the degradation rate was27.1～43.3% at10th week; the best formula that is SH0.09g, KGM2.75g, lactic acid0.9ml, microwave heating for4min and crosslink4days was confirmed by comprehensive analysis on above results.It was demonstrated that the film cell adhesion is weak, but the membrane in the rabbit did not lead to abscesses, tissue degeneration, necrosis, and abnormal proliferation according to the cell culture experiment in vitro and subcutaneous experiment in vivo. So that the film was proved have certain biocompatibility. Trough experiments found that the degradation rate of the film is lower, such as the optimal formula membrane degradation in PBS solution was35.8%at10weeks, the degradation rate amplitude slow down compared with the pure SH gel. SH/KGM blend films have great attached performance after moisture absorption because it’s higher water absorption, and have certain tenacity after absorbed water, so it can assure that not have brittle fracture during use.2. Secondary, Sodium hyaluronate/Chitosan (CS)/Konjac glucomannan blend film were studied in this paper, which used Sodium hyaluronate and Chitosan as raw materials, Konjac glucomannan and acetic acid as modifier and an additive, respectively. And then through fully dissolved, deaeration in vacuum conditions, film casting, crosslink the film after dried at last. On this basis, to judge the influence of KGM content for the blend films property.The SH/CS/KGM blend films were pale yellow, and one side is rough, and another side is relatively smooth. And the films were found have great miscibility according to transmittance and SEM. It was demonstrated that the films have good biological compatibility because it can effectively support cells growth and the cell have good morphology. But degradation of the films is varied5.78%to6.47%, which is showed that the films’ degradation is lower.The film toughness was improved by adding of KGM, which solved the shortcoming of SH/CS blend films’toughness shortage. The result shows that the films have higher water absorption than SH/CS blend films. As we all known higher water absorption can adsorb the fluid of the tissue which at injury location, and reduce inflammation. But as the adding of KGM, the biocompatibility has reduced slightly. |
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