Drug Transport And Its Biological Effects Research Of Nanometer Diamond

Chemotherapy has been used widely and is one of most effective meansin present cancer therapy. However, the most of clinically used anticancerdrugs are low molecular weight compounds that show a short half-life inbloodstream and a high overall clearance rate. Only a small part of the drugafter drug administration will be transported to the tumor tissue，so the tumorcells have poor efficacy and strong toxicity. Recently nanodiamond has wideapplication in carrying protein, DNA and drug for their noncytotoxic nature.In this paper, a new kind of drug delivery system (ND-DOX) was prepared,using HepG2as a model cell，we find ND-DOX nanoparticles remain gooddrug activitycan、 have potential slow/sustained released capabilitiescomparing with DOX and can kill the cells effectively by MTT、laserscanning confocal microscope and flow cytometry. Based on vitro data, wemade animal experimen to study whether ND-DOX could act as potentialantineoplastic agents in vivo application. An interesting phenomenon wasfound that survival prolonging probability for ND-DOX treatedtumor-bearing mice is about75%. More exciting, survival prolongingprobability by bare ND-treated achieve41%, while DOX-treated is only19%. Histopathological analysis showed that nanodiamond and the complexhave not apparent side effects on kidney, liver, and spleen tissue fromtreated mice, while the toxic effects of doxorubicin on kidney and liver areobvious. Therefore, ND can be a potential material of prolonging thesurvival life span applications by tumor-bearing mice abdominal wallinjected, and ND-DOX can act as a nano-drug with a promising, enhancingchemotherapy efficacy and safety. The same time, we use doxorubicin (DOX) as a drug model,transferrin(Tf) adsorbs doxorubicin by physical adsorption. Through MTT、laser scanning confocal microscope and flow cytometry, we get thatTf-DOX complexes enter cells through clathrin-mediated pathway having anenergy-dependent manner, while free adriamycin is passive diffusion intothe cells. Also Tf-DOX complexe has higher lethal than doxorubicin since itcan target to tumor cells.