Copper Complexes Have Listed Bacteriostatic Activity And Plant Growth Regulating Effect Research

Tiazole fungicides have double functions in the agriculture. In addition to be the efficient and harmless specific fungicides, they also function as non-traditional plant growth regulators which protect plants against various environmental stresses. In this research, the complex of hexaconazole, one frequently-used tiazole fungicides, with copper acetate have been synthesized. The functionality of the hexaconazole-Cu complex is has been investigated by antifungi test and plant growth regulating test after the characterization of complex composition and molecular structure.The results are as follows:(1) The composition and structure parameters of the compound are determined by elemental analysis, IR spectrum and thermogravimetric analysis. The results show that the hexaconazole-Cu complex is composed of one copper acetate, two hexaconazole and two acetates, so the molecular formula is [Cu(C14H17N3OCl2)2(CH3COO)2]. The schematic structure of the compound is presented in chapter2.(2) Antimicrobial activity of the compound and original hexaconazole to Saccharomyces Cerevisiae are tested at different concentrations. The results indicat that the hexaconazole-Cu complex displays superior sterilization capability than that of hexaconazole. The inhibition zone diameters of0.05mmol/L hexaconazole-Cu complex is1.48times larger than that of0.10mmol/L hexaconazole.(3) The hexaconazole-Cu complex inhibits wheat seedlings growth, leading to increased root weight. The wheat seedlings treated by0.05mmol/L complex has stem length of49.33%lower than that of the control, and its root weight is1.599times heavier than that of the control. Comparing with hexaconazole, hexaconazole-Cu complexe does not demonstrate remarkable effect on plant height and root weight (P<0.05).(4) The hexaconazole-Cu complex changes the physiological environment of wheat seedlings, which contributes to the remarkable plant growth regulating function. After being treated with0.03mmol/L hexaconazole-Cu complex, the activity of SOD, POD, the concentration of GSH and chlorophyll of wheat seedling increase24.65%,61.06%,30.41%and59.04%respectively relative to those of the control. Comparing to the control, the concentration of MDA of0.01mmol/L complex treated wheat seedling is33.33%lower. The soluble protein content increased52.87%relative to the control under0.05mmol/L complex treatment. This research indicates that the hexaconazole-Cu complex is significantly better than hexaconazole on improving POD activity and restraining MDA accumulation. The activity of POD of0.03mmol/L complex treated wheat seedling is29.07%higher than that of wheat seedling treated with0.06mmol/L hexaconazole. The concentration of MDA decreased26.67%under0.01mmol/L complex treatment relative to0.02mmol/L hexaconazole.(5) Proline is an important osmoprotectant for plant cells under osmotic stress imposed by NaCl. The hexaconazole-Cu complex depicts significant influence on the proline content of wheat seedlings. In the presence of200mmol/L NaCl, The proline content of0.03mmol/L complex treated wheat seedlings is13.68%higher than that of hexaconazole treated, and is58.45%higher than that of the control. The impact of triazole-metal treatment on the expression of the genes encoding△1-pyrroline-5-carboxylate synthetase (P5CS) and△1-pyrroline-5-carboxylate reductase (PC5R) was investigated by RT-PCR analysis.The results show that the hexaconazole-Cu complex enhances the accumulation of the P5CR and P5CS transcript. After being treated by complex for6h in the presence of100mM NaCl, the P5CS transcript approachs steady state, with a relatively higher transcript level than that of hexaconazole treated.