Wheat - Pastel Suppress A Wheatgrass New Some Double Diploid Chromosome Identification

As an important agricultural crop of China, wheat is essential to Chinese foodsecurity, due to bacteria physiological continuously updated, the disease resistance ofcommon wheat is poor. In past50years，the production of wheat in Yangtze river basinhad falled sometimes. So the main work of the wheat breeders is transferring the genewhich is from the wheat Related Species and has the ability to resist to the wheatdisease to common wheat. Thinopyrum which is related sepecies of common wheat,has some genes which are resistance to wheat powdery mildew, stripe rust, leaf rust,stem rust and yellow dwarf virus. Thinopyrum also have ability to resist lodging coldand drought, which can be as an excellent gene pool of wheat and widely be used inwheat breeding research.Thinopyrum intermedium (Th. Intermedium2n=42, genome JJJ~sJ~sStSt) can haveresistance some diseases of wheat. Which is one of successful species in wheatbreeding. Thinopyrum intermedium ssp.trichophorum is a subspecies of Thinopyrumintermedium, which has many excellent resistance genes of Thinopyrum intermedium.In North Amreica, Thinopyrum intermediun ssp.trichophorum is widely cultivated as apasture. So this species can be considerd as the resource of improving wheat quality.Wheat-Thinopyrum intermedium ssp.trichophorum amphidiploid or part of theamphidiploid is a way to transfer the resistance disease gene to common wheat,Therefore, firstly we should identify a series of wheat-Thinopyrum intermediumssp.trichophorm amphidiploid or part of the amphidiploid from offsprings of Wheat-Thinopyrum intermedium ssp.trichophorum.In this study, we use molecular markers to screen the offspring and get the newmaterial which has the genome of Thinopyrum intermedium ssp.trichophorum, andPCR makers,multicolour fluorescence in situhybridization (mcGISH) and resistanceidentification to study the four Wheat-Thinopyrum intermedium ssp.trichophorumpartial amphidiploid L451-3-1, Y69, Y70,1508-2-1. And we find the chromosome ofwheat occur translocation. These four lines are relatively stable, foreign gene istransferred into lines which get disease resistance. Using mcGISH, we can clearly know translocations and addition of foreign chromosome.In summary, by mcGISH and protein electrophoresis, this research cansuccessfully identify the chromosome of the four materials. These materials show theability of disease resistance, In the future, we should observe the offspring of thesematerials, in order to understanding the impact of internal wheat translocation.