State Of The High Sugar Rhepo Rat Retina Muller Cell Apoptosis And The Nf-kappa B Expression

Objective:To observe the protective effect of Recombinant human erythropoietin (rhEPO) on the apoptosis of cultured rats Muller cells induced by high glucose in vitro and the possible mechanism.Methods:Muller cells were seprated and pirified from the retinas of 3d SD rats and were randomly divided into control group, high glucose group and the rhEPO-treated group with different concentrations. After 48 hours, The apoptosis of Muller cells was detected by TUNEL. The expression levels of NF-κB protein was detected by semi-quantitative immunocytochemistry.Results:There were only a few apoptotic cells found in control group.The number of apoptotic cells in the rhEPO-treated groups was significantly lower than that in the high glucose group (P<0.01), and which was positively related to concentration. Application of exogenous rhEPO had a protective effect on Muller cells with injury of high glucose, adding 5-40U/ml rhEPO treatment could effectively up-regulate the expression of NF-kB (P<0.01).Conclusion:rhEPO treatment can inhibit the apoptosis of Muller cells induced by high glucose injury, and its inhibition effect is related to the rhEPO concentration. Up-regulating the expression of NF-kB may be one of the mechanisims.