| 1. Research purposeBy observing the polygonatum sibiricum extract and swimming training in mice quadricepsaerobic oxidation of the marker enzyme for energy systems(SDH and MDH), anaerobicoxidation of marker enzyme(LDH) and muscle glycogen,and to explore the impact of thepolygonatum sibiricum extract on energy metabolism in the different states, and taking thePolygonatum extract on exercise performance. Experimental basis for the development andutilization of Polygonatum exeract as sports nutrition supplements.2. Research MethodsSelect8week old male Kunming mice the number of128, weighing control in20to24g,provided by the Experimental Animal Center of Shandong Lukang Pharmaceutical. Animal roomventilation well, natural lighting, and strict control of the animal room temperature and humiditywere23-28℃and40%-65%. Animal feed by the Experimental Animal Center of ShandongLukang Pharmaceutical, experimental animals drinking water for cold water. The experimentalmice were housed separately, eight per cage. One week before the start of the experiment, let themice were adaptive feeding in the animal room to become familiar with the enviroment.Adaptive feeding stage the mice were randomly divided into four major groups, including thecontrol group,exercise group, medication group, exercise+medication group. Each large groupof32, after4weeks swimming training experimental mice in each group were sacrificed state isdivided into four sub-group of the state: quite state group, exercise one hour group, exhaustiveexercise group and after exhaustive exercise recovery12h group. Broken the cervical to kill themice and taken the quadriceps to determinate the muscle glycogen content, succinatedehydrogenase(SDH), malate dehydrogenase(MDH), lactate dehydrogenase(LDH).3. The experimental results3.1Exhaustive swimming time, exercise+medication group were obviously longer than theother three groups (P <0.01), exercise group and menication group were obviously longer thancontrol group(P <0.01).3.2Quiet state, muscle glycogen content, exercise+medication group was significantly higherthan other groups (P <0.01), exercise group and medication group were significantly higher thancontrol group (P <0.01); SDH activity, although exercise group was the hightest no significantdifference between groups; MDH activity, exercise+medication group was significantly higherthan control group (P <0.01), and significantly higher than exercise group and medicationgroup(P <0.05); LDH activity, exercise+medication group was significantly higher than controlgroup (P <0.05). 3.3Exercise1hour, muscle glycogen content, exercise+medication group was significantlyhigher than control group, exercise group and medication group (P <0.01),; SDH activity,controlgroup were significantly higher than exercise group and medication group(P<0.05),and weresignificantly higher than exercise+medication group(P<0.01); MDH activity, the other threegroups were significantly higher than control group(P<0.01); LDH activity, exercise+medication group was significantly higher than control group (P <0.05).3.4Exhaustive instant status, muscle glycogen content, exercise+medication group wassignificantly higher than the control group(P<0.01),and were significantly higher than exercisegroup and medication group(P<0.05); SDH activity, control group was significantly higher thanexercise+medication group(P<0.01),and were higher than exercise group and medicationgroup(P<0.05); MDH activity, exercise+medication group was significantly higher than controlgroup(P<0.01), exercise group and medication group were significantly higher than controlgroup(P<0.05);LDH activity, exercise+medication group was obviously higher than controlgroup (P<0.05).3.5Exhaustive recovery12h, muscle glycogen content, the other three groups were significantlyhigher than control group(P<0.01); SDH activity, control group was significantly higher thanmedication group(P<0.05), exercise+medication group was significantly lower than theexercise group and the control group (P<0.01); MDH activity, exercise+medication group wassignificantly higher than medication and exercise group(P<0.05),and was significantly higherthan control group (P<0.01); LDH activity, exercise+medication group was significantly higherthan control group (P <0.05)。4. Conclusion4. Conclusion4.1The exercise training can improve quadriceps muscle glycogen content in mice andcontribute to the restoration of muscle glycogen synthesis process, increase the state ofexhaustion immediately MDH activity and increased aerobic oxidative capacity, and improveathletic ability.4.2Polygonatum extract can improve quadriceps muscle glycogen content in mice andcontribute to the restoration of muscle glycogen synthesis process, increase the immediate andrecovery after exhaustive aerobic metabolism under12h enzymes MDH, Polygonatum extractcan slow abnormal elevation of SDH activity within the cytoplasm of muscle cells duringexercise, suggesting that Polygonatum extract improve athletic ability.4.3Both polygonatum sibiricum and exercise can slow the rate of decline of muscle glycogenduring exercise, improve the recovery rate of muscle glycogen after exercise and promote theactivity of the MDH in the quiet state. LDH activity has also improved, it shows that the Polygonatum extract has a certain influence in the ability of anaerobic metabolism. |
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