Small Guobo Fall Back Into The Leaf Quality Standard And Fingerprint Study Of Different Regions

Macleaya microcarpa (Maxim) Fedde is a species of the genus Macleaya R. Br., Papaveraceae sp..It is widely distributed in Shanxi、Gansu、Hubei in China.The main active ingredients are protopine, allocryptopine, sanguinarine, chelerythrine.It has important value in anti-bacterial, anti-tumour, improve liver function and Enhance immunity and so on.At present,the herb extract is largely used in breeding production as a Veterinary Drugs and biological pesticide.For ensuring the leaf quality of Macleaya microcarpa(Maxim)Fedde,the main contents and results were divided into three sections as follows:(1) The quality standard research of the leaf of Macleaya microcarpa (Maxim) FeddeThe topic of the experiment studies is leaf of Macleaya microcarpa (Maxim) Fedde.According to technical requirements in the appendix in China Pharmacopoeia (I,2010ED),systematically to study the topic through source, characters, identification, inspection (moisture, ash content, extract, heavy metal detection),the content determination and so on.The research set up a best extraction method and High performance liquid chromatography (HPLC) analysis method for protopine, allocryptopine, sanguinarine, chelerythrine in the content determination, and provide a reference of qualifying control methods for medicinal formulation to provide a reference. (2) The HPLC fingerprint research of the different origin of Macleaya microcarpa (Maxim) Fedde.The experiment study the HPLC fingerprint conditions and data;establish the standard fingerprint and obtain the similitude degree between the leaf of Macleaya microcarpa (Maxim) Fedde in17different origins and the standard fingerprint more than0.9.(3) Comparison of contents of four alkaloids in leaf of Macleaya microcarpa (Maxim) Fedde and Macleaya Cordate (Willd.) R. Br. HPLC method was used for the determination of four alkaloids in leaf of Macleaya microcarpa (Maxim) Fedde and Macleaya Cordate (Willd.) R. Br..We found that the content of protopine and allocryptopine in leaf of Macleaya Cordate (Willd.) R. Br. is more than in Macleaya microcarpa (Maxim) Fedde, but content of sanguinarine and chelerythrine.Finally, we establish the leaf quality standard and standard fingerprint of Macleaya microcarpa (Maxim) Fedde, distinguish Macleaya Cordate (Willd.) R. Br. that is easily confused, and provides the basis for evaluating the leaf quality of Macleaya microcarpa (Maxim) Fedde more accurately and comprehensively.