Mir - 181 - C Gene Methylation In Gastric Cancer Tissue

Objective:To discuss pathogenesis of gastric cancer to find gastric carcinogenesis markers through comparing the miR-181c gene promoter methylation status and the changes of Pre-miR-181c、NOTCH4and KRAS gene expression between control and Gastric cancer group.Methods:We selected60cases of fresh endoscopic biopsy specimens, including gastric cancer30cases(18males and12females, mean age58years) in gastric cancer group, and30cases(14males and16females, average age54years) in control group(normal or superficial gastritis). Then detected the miR-181c gene promoter methylation status by methylation-specific PCR method (MSP), and the gene expression of Pre-miR-181c、 NOTCH4and KRASby RT-PCR.Results:The rate of Hypermethylation in miR-181gene promoter region in gastric cancer group was significantly higher than in the in control group; gene expression of Pre-miR-181c was decreased significantly(P<0.05) compared with control group and gene expression of NOTCH4and KRAS were significantly higher than the control group (P <0.05).Conclusion:MiR-181c may not express or express in a lower level through methylation and regulate expression of target genes NOTCH4and KRAS, which paly a important roles in gastric carcinogenesis. The methylatin status of miR-181c may become marker of gastric carcinogenesis and progression.