| Objective:This experiment through the establishment of SD rat atrial fibrillation cell model, with fluorescence PCR technique for the observation of tanshinone Ⅱ-A sodium sulfonate on atrial fibrillation atrial muscle cell membrane potassium ion channel Kv1.5gene expression in rats. Study on tanshinone Ⅱ-A sodium sulfonate on rat atrial electrical remodeling in atrial fibrillation and atrial muscle cell membrane potassium ion channels, which reveal the prevention and treatment of atrial fibrillation mechanism.Methods:Use of calcium chloride and acetylcholine in rat tail vein administration of atrial fibrillation animal model establishment. Screening40rats with typical atrial fibrillation electrocardiographic manifestations, and the rats were randomly divided into4groups. They were model group, atrial fibrillation of tanshinone Ⅱ-A sodium sulfonate, amiodarone treatment control group, normal control group. After the success of each model, extracting the rat heart, separation of atrial myocytes and simultaneously placed in each drug culture medium. Tanshinone Ⅱ-A sodium sulfonate treatment group and amiodarone control group cells were placed in liquid treatment.24hours later, determination the gene expression and ultrastructural changes of atrial muscle cells about each rat atrial myocytes on Kvl.5potassium channel.Results:The model group compared with normal group, atrial muscle cells in Kvl.5potassium channel gene expression was significantly decreased (P<0.05) compared to the normal group. Tanshinone Ⅱ-A sodium sulfonate, amiodarone treatment control group compared with the model group, could significantly increase of atrial fibrillation in rat atrial myocytes potassium channel Kvl.5gene expression level (P<0.05). Tanshinone Ⅱ-A sodium sulfonate treatment group and control group of amiodarone for atrial fibrillation in rat atrial myocytes potassium channel Kvl.5for improved gene expression levels compared to control group, amiodarone is better than that of treatment of tanshinone Ⅱ-A sodium sulfonate treatment group (P<0.05). At the same time, the model group compared with the normal group, atrial muscle cell ultrastructure of serious injury, tanshinone Ⅱ-A sodium sulfonate group and control group treated with amiodarone for atrial muscle cell ultrastructure of improved significantly, especially in the observation group highlights.Conclusion:Tanshinone Ⅱ-A sodium sulfonate treatment group can significantly improve atrial fibrillation rat Kvl.5potassium channel gene expression is downregulated by atrial electrical remodeling, resulting in the prevention and treatment of atrial fibrillation occurred, but with the extensive use of class III antiarrhythmic amiodarone, role less weak. The traditional Chinese medicine tanshinone II-A sodium sulfonate on atrial fibrillation prevention and treatment has broad prospects, also provides clinical and use of tanshinone treatment with amiodarone for atrial fibrillation effect will be far greater than the single Chinese drug use, and it reduces the side effects which caused by using amiodarone in the treatment of atrial fibrillation simply. |
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