Born Fleece-flower Root Cooked In Mechanism Research

Polygonum multiflorum, also known as Polygonum, Polygonaceae plant Polygonum multiflorum Thunb. Perennial twining vine. The medicinal parts are generally the dry tuber. Chinese medicine clinical medicine, Polygonum use their remaining cooked the other. Polygonum multiflorum is bitter to the users of the original ingredients, their raw materials, the color yellow and white, the effectiveness of multi-laxative, detoxifying swelling in the history of ancient Chinese pharmacy, the Tang Dynasty and before common use of its raw products, treatment of senile intestinal constipation, dry, rubella, scrofula; Polygonum multiflorum for clinical use of the products after processing methods, sweet, skin color black, the cross section color brown, black beard and hair, strong bones and muscles, the effect of the Bushenyijing in China’s Polygonum multiflorum drug, Polygonum multiflorum products use up to the use frequency of the raw products, indications and more premature graying, blood deficiency, fatigue, high cholesterol and the weak waist.Progress for many years in medicinal chemistry and pharmacological studies in Polygonum multiflorum, also a number of articles reported in the literature to recent research. However, access to large amounts of Polygonum multiflorum literature, Polygonum multiflorum quality control evaluation of a single active chemical components of the index for judging the show has been one-sided and inadequate evaluation of quality of TCM, the same time, Polygonum multifleruin medicinal chemical composition, its pharmacological effect described is relatively independent of both, often not effectively reveal the law of the pharmacodynamics of the chemical composition, on the contrary findings of the pharmacological effect is difficult to reflect the change of chemical composition of the substance group, which gives the material determines the efficacy, effectiveness of contrasting material change the interpretation of correlation difficult. Therefore, in order to explore the relationship between the raw goods and products of Polygonum multiflorum efficacy chemical composition and pharmacodynamics (spectrum effect relationship), traditional Chinese medicine quality control system to provide a theoretical basis Fingerprinting and Chinese medicines associated with the establishment of chromatographic fingerprint technology and The data support. Research methods and results are as follows:1Polygonum multiflorum raw materials, the HPLC method a different processing time products of the methanol extract and water solution fingerprint. Polygonum multiflorum the crude methanol extract fingerprint common pattern according to the commercial origin of the12batches of samples collected from domestic12herbs calibration peaks32,22,25,26,on the27th four refers to the peak spectrum Total characteristic peaks, peak shape and sharp, good separation and peak symmetry, peak information. Polygonum multiflorum total water fluid mode peak relative to alcohol solution fingerprint peak less. Cooked up the process, the change of total peak significant difference between the raw product and1h, the difference in composition between2h,3h,4h after each component change stabilized, the composition change was no significant difference, suggesting that Polygonum multiflorum processing technology in The concocting time control in4h range is appropriate. Found between the raw products and processed products of the original peaks disappear, while new component peaks; calibration of the31peak composition as chrysophanol attribution to the chromatographic conditions, to detect the content of0.32%, with the follow-up study depth and medicine standards improve, make provisions on the the chrysophanol composition testing standards and specification.Polygonum multiflorum Health diarrhea cooked complement pharmacological effects, the establishment of relevant pharmacodynamic model experiments. Purgative effectiveness of Polygonum multiflorum raw medicinal herbs, medicinal properties can be qualitatively and quantitatively reflect the medicinal effects of Polygonum multiflorum raw goods diarrhea, based on mice feces color point method with carbon ink Propulsion Laboratory, discovered the role of2b,3h products without system diarrhea, while The4h products have significant antidiarrheal effect (P<0.05) compared with the saline group, there were significant differences statistically significant. Reported in the literature in recent years, bound anthraquinones from Polygonum multiflorum play diarrhea under the material basis for the role, to play a purgative mechanism to stimulate the intestinal wall motility, promoting defecation of mice and rabbit intestinal experiments confirmed that combines anthraquinone Polygonum multiflorum Health product groups, the lh products group,2h group of products on isolated rabbit intestinal muscle contraction tension with the saline group have significant difference (P O.001), no significant difference after3h products group, the process to4h best.2Polygonum multiflorum products efficacy for the benefit function of the body, the experiments by human embryo lung diploid cells (HEL) anti-aging experiments to verify the tonic effect of Polygonum multiflorum products. Health products Polygonum multiflorum,1h products Polygonum cells the decline of efficiency, similar to the decline of efficiency of the normal cells, the SOD activity among the three useless significant difference (P>0.05),2h,3h,4h products Polygonum multiflorum and blank control group cells to improve the effectiveness of the activity of SOD,4h product Polygonum multiflorum highest SOD activity, with the blank group were more significant differences (P<0.001), indicating4h products Polygonum multiflorum delay the decline of the role of human embryonic lung cells, increase SOD activity in HEL cells.3With chemometrics computer simulation evaluation of systems analysis methods Polygonum multiflorum Health products associated with different processing time product water liquid extract on HPLC fingerprint of raw and cooked different efficacy. To make the various elements of the system through the gray relational analysis between the factors in the development process relative changes in line, the correlation between the factors; on the contrary, the correlation is small. Basis associated with the degree to determine the components of the order of the size of the contribution of diarrheagenic role is:2>7>30>1>3>1>29>27>32>5>6>28>8>16>4>26>22>11>14>*2>12>9>21>24>25>*3(number of peaks as the peaks represent chemicals). Of each component peak anti-aging effects the contribution of order:11>14>8>28>5>2>29>1>3>32> 6>7>1>30>2>4>27>26>22>16>12>*3>21>24>25>9(to the number of peaks as the peaks represent chemicals).Data mining of traditional Chinese medicine Polygonum multiflorum based drug polypharmacy law. Pharmacopoeia side by side and you want prescription drug compound to collect the relevant records Polygonum multiflorum, the establishment of Polygonum multiflorum compound database, the use of frequency analysis, cluster analysis and association rule mining compound data. Involving major drug in the treatment of liver or kidney essence and blood22prescription and20species were used frequency of121times, Ziyin drug blood to close the three categories of astringent medicines and tonics, association rule analysis of four drugs on the18groups of drugs; fill fine prescription, involving17kinds of main drug in the treatment of kidney, the total frequency of64points kidney dampness drugs, tonic medicines, two class association rules analysis of the five drug groups; in liver wind18prescription treatment of diarrhea, frequency of157points tonic medicine, liver Qi drug, relieving drugs and Expelling Wind wet drugs in four categories, the correlation analysis the drugs associated to a group of drugs23Polygonum multiflorum treatment Qingrejiedu prescription, involving16kinds of main drug, a total of92times the frequency of antipyretic points diarrhea, relieving drugs, gunpowder and the heat of the heat diarrhea relieving drugs in four categories, association rules2drug and21drug groups; in the treatment of intestinal dry constipation Fang, involving14kinds of major drug use frequency16times Ziyin drug Qingre laxatives and diarrhea prescription three categories, association rule analysis13drug, six drug groups;14kinds of major drug prescription in the treatment of malaria, involving a total frequency of30points qi and yin tonic, cut malaria drug that liver Qi stagnation drugs in three categories, association rules There is a drug and20drug groups.