| The research aims at observing the effect on hypertension, Ang Ⅱ, TGF-β and p38in SHR by reinforcing-reducing manipulation with ELESA, RT-PCR and WB. It reveals the validity and diversity between reinforcing-reducing manipulation by twirling and explores the impact of the interventions of different twisting tactics of TGF-β and p38expression in SHR vascular remodeling. It also analyzes how twirling reinforcing-reducing manipulation can reduce blood pressure through p38/MAPK. It provides scientific demonstration for the mechanism of reinforcing-reducing manipulation by twirling.MethodParticipants were75spontaneously hypertensive rats,15WKY rats in clean grade, male,9-week-aged, weighing180～220g. SHR were randomly divided into5groups (n=15), reducing group, reinforcing group, needling group, Taichong pressing group and model group and15WKY rats as a blank group. Reinforcing manipulation group was treated by needling "Taichong" point with twirling reinforcing manipulation and reducing group was treated by needling "Taichong" point with twirling reducing manipulation. Needling group was treated by needling "Taichong" point without manipulation. The Taichong pressing group was treated by perssing "Taichong" point on both sides rhythmically using a needle handle. The model group and blank group were treated by the same crawl stimulation. Each manipulation lasted for1minute, in a frequency of60beats/min. needle retaining for20minutes and were completed by the same person. The treatment was operated once per day in a14-day period. The systolic blood pressure(SBP) was measured in all of the groups on4th.7th,10th,13th day. At the end of the treatment. AngⅡ were measured by means of ELISA, the mRNA expression of TGF-β were detected by means of RT-PCR. p38protein kinase activity were measured by means of WB.Result1The systolic blood pressure of the ratsThe systolic blood pressure of SHR group had no significant difference (P>0.05) before acupuncture treatment. It was significantly higher than blank group (P<0.01). After14days of treatment, the systolic blood pressure of reducing group were different from the reinforcing group (P<0.05) and significantly different from other groups (P<0.01) and the systolic blood pressures of all the groups were not significantly different than before(P>0.05). The reducing group and the reinforcing group were difference of non-acupuncture group(P<0.05) and no significant difference of needling group(P>0.05) and no difference before the treatment (P>0.05). The needling group were significant difference of reducing group, blank group(P<0.01) and no significant difference of reinforcing group, non-acupuncture group (P>0.05). The systolic blood pressure of Taichong pressing group and model group were different before acupuncture treatment(P<0.05). The systolic blood pressure of blank group had no significant difference (P>0.05).2AngⅡThe Ang Ⅱ of model group was lower than blank group (P<0.05). The Ang Ⅱ of reducing group was highly significant difference of the needling group and model group and blank group (P<0.01) and was difference of reinforcing group and Taichong pressing group (P<0.05). The Ang Ⅱ of reinforcing group was highly significant difference of needling group and model group(P <0.01), was difference of Taichong pressing group (P<0.05) and no significant difference of blank group (P>0.05). There was no significant difference between Taichong pressing group and needling group and model group and blank group (P>0.05). There was also no significant difference between needling group and model group and blank group (P>0.05).3p38The p38of model group was lower than blank group (P<0.05). The p38of reducing group and Taichong pressing group were difference of other groups (P<0.05).But the p38of reducing group was difference of reinforcing pressing group(P<0.0l). The p38of reinforcing pressing group was slightly higher than Taichong pressing group(P<0.05) and was difference of other groups(P0.05). There was also no significant difference between needling group and model group and blank group (P>0.05).4TGF-βThe expression of TGF-β mRNA in model group was lower than in blank group (P<0.05). The expression of TGF-β mRNA was stronger in reducing group than other groups. There was difference between reducing group and needling group and model group (P<0.05) But there was no difference between reducing group and reinforcing group. Taichong pressing group and blank group(P>0.05). The expression of TGF-β mRNA in reinforcing group was difference of needling group (P<0.05) and no difference of model group(P>0.05). The expression of TGF-β mRNA in reinforcing group was slightly higher than in Taichong pressing group and blank group but there was no difference between them(P>0.05). The expression of TGF-β m RNA in Taichong pressing group was lower in needling group(P<0.05) and in model group and blank group(P>0.05). There was also no significant difference between needling group and model group and blank group (P>0.05).Conclusion1Twirling reducing manipulation could reduce the systolic blood pressure of the9-week-aged SHRs. Twirling reducing manipulation is better than reinforcing manipulation. The twirling reinforcing manipulation has no significant effect on reducing systolic blood pressure.2The blood pressure regulating mechanism of twirling reducing manipulation could be related with the p38/MAPK signal pathway. This process might function through reducing the content of Ang, therefor inhibiting the p38signaling pathway mediated by TGF-β.3There were diffrences in the effects among the twirling reinforcing-reducing manipulations that used in this experiment. |
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