Blood Plug On Game Focal Cerebral Infarction In The Rat Hippocampus Nogo - A, The Influence Of Syn, Psd - 95 Expression

Objective:To observe effect of xuesaitong on the expression of Nogo-A、SYN、PSD-95in rat hippocampus at different times in SD rat model of middle cerebral artery occlusion, on the other hand, to explore the dose-effect relationship of XueSaiTong.Methods:The maleSD rats were divided into control group and operation group randomly. The model of middle cerebral artery occlusion(MCAO) was established by improved Longa EZ method and then observing the change of nerve functions, pathomorphology and expression of Nogo-A、SYN、PSD-95in rat hippocampus at selected different time point.Results:1.Normal stateAll the operation groups of rats are less active within24hours after the operation. The furs is gloom。The reaction is blunt,and difficulties in eating and drinking.7days after operation, all the operated rats have recovered in mental state, reaction and activity. The XueSaiTong group and Ni Mo Di Ping group had a better effect compared to the control group in recovery.14days after operation, the rats in each group are quickly recovered. The appetite is normal, and activity is well.28days after operation, all the rats in operation groups are as well as normal animals which have good mental state, appetite and activity during the experiment.2.Score of nerval functionThe score of nerval function of operation group decreases with time course. The nerval function of model group is severely damaged at5hours after operation.48hours after operation, the damaged nerval function is partially recovered and the score is decreased.72hours after operation, the score of model group is obviously higher than XueSaiTong group and Ni Mo Di Ping group.3. Pathomorphology observationHE staining indicates that the neurons of rat hippocampus in normal group rats have a large number and have a neat arrangement. The neurons have integrated and big cell structures with big and round nucleus and obvious nucleolus. However, the neurons of rat hippocampusin model group are disordered with indistinct boundaries. The gap between neurons is larger. Normal cell structure is disappear and many neurons are become pyknosis and necrosis. Compared to control group, XueSaiTong group and Ni Mo Di Ping group are improved in pathomorphology changes. The neurons have neat arrangement and integrated cell structure and less number of shrined ones. Also, the large dosage of XueSaiTong group has better effect compared to the small dosage group.4.The effect of XueSaiTong on expression of Nogo-A in rat hippocampusThe expression of Nago-A in model group rats is upregulated after7,14and28days of MCAO operation. Large dosage group of XueSaiTong rats have lower Nogo-A expression level than the small dosage group.5.The effect of XueSaiTong on expression of PSD-95and SYN in MCAO ratsThe operated rats have an increasing on expression of SYN and PSD-95with time. At different time points, the expression of PSD-95and SYN in large and small dosage groups of XueSaiTongare higher than model group.7days after MCAO operation, the expression of PSD-95and SYN in model group are both lower than normal group. The expression of PSD-95and SYN in xuesaitong groups is higher than both normal and model group at14d and28d after MCAO operation. Large dosage group of XueSaiTong have higher SYN and PSD-95expression level than the small dosage group at day28.6. The conclusion1) XueSaiTong could promote nerval function recovery after cerebral ischemiaso as to promote brain rehabilitation.2) XueSaiTong could efficiently reduce the expression level of inhibition factor Nogo-A.3) XueSaiTong could promote synapses rehabilitation after cerebral ischemia.4) Large dosage XueSaiTong works better than small dosage group.