| Tianma toufengling capsule composed of ten Chinese herbs:Rhizoma Gastrodiae, Radix Rehmanniae, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Flos Chrysanthemi Indici, Herba Visci, Radix Achyranthis Bidentatae, Cortex Eucommiae, Radix Scrophulariae and Ramulus Uncariae Cum Uncis, is one of traditional Chinese medicine recipe for nourishing yin, dispelling wind and strengther muscles and bones. The current quality standard only determination gastrodin and ferulic acid. In this thesis, HPLC method was employed in quality control of index components in tianma toufengling capsule and also in studying pharmacokinetics of gastrodin in rat.HPLC methods were established to control the quality for Tianma toufengling capsule. Gastrodin, catalpol, buddleoside, harpagoside, cinnamic acid, rotocatechuic acid, syringin, chlorogenic acid, caffeic acid, ferulic acid and acteoside were determination in tianma toufengling capsule. The calibration curve was linear within the range of 18.60-186.0μg·mL-1 for gastrodin (r=0.999 8, n=6),4.600-46.00μg·mL-1 for catalpol (r=0.9997, n=6), 11.0-110μg·mL-1 for buddleoside (r=0.999 2, n=6),4.60-46.0μg·mL-1 for harpagoside (r= 0.999 6, n=6),4.20-42.0μg·mL-1 for cinnamic acid (r=0.999 6, n=6),2.400-24.00 ug·mL-1 for rotocatechuic acid (r=0.999 6, n=6),5.200-52.00μg·mL-1 for syringin (r= 0.999 7,n=6),26.60-266.0μg·mL-1 for chlorogenic acid (r=0.999 5, n=6),0.400-4.00μg·mL-1 for caffeic acid (r=0.9994, n=6),2.10-21.0μg·mL-1 for ferulic acid (r=0.999 6, n =6),0.600-6.00μg·mL-1 for acteoside (r=0.999 6, n=6). The average recoveries were 101.0%(RSD=1.2%, n=9) for gastrodin,101.4%(RSD=1.7%, n=9) for catalpol,101.1% (RSD=1.9%, n=9) for buddleoside,100.3%(RSD=1.6%, n=9) for harpagoside,101.8% (RSD=1.7%, n=9) for cinnamic acid,101.0%(RSD=0.9%, n=9) for rotocatechuic acid, 100.3%(RSD=1.9%, n=9) for syringin,101.5%(RSD=1.4%, n=9) for chlorogenic acid, 101.0%(RSD=1.9%, n=9) for caffeic acid,101.1%(RSD=1.3%, n=9) for ferulic acid, 99.6%(RSD=1.6%, n=9) for acteoside. The method is simple, accurate, reproducible with a strong specificity and be used for the quality control of tianma toufengling capsule.The method of determine gastrodin in rat plasma after i.g. administration of Tianma toufengling capsule. The HPLC separation was achieved on a Diamonsil C18(200 mm×4.6 mm,5μm) column at 35℃. The mobile phase consisted of acetonitrile-aqueous (2:70, v/v). The flow rate was 1.0 mL·min-1 and the UV detection wavelength was set at 221nm. The calibration curve of Gastrodin was linear in the concentration range of 25-1000 ng·mL-1(r= 0.998 1). The RSDs of inter-and intra-day were less than 6.5%. The average recovery of Gastrodin was more than 69.4% and 70.4% for the internal standard.The main pharmacokinetics parameters were as follows:Cmax was 1100 ng·mL-1, t1/2 was 0.39 h, AUC0â†'t was 1365μg·h·L-1, AUC0â†'∞was 1377μg·h·L-1. The method is simple, special, accurate and reproducible, which was suitable for the preclinical pharmacokinetics study of gastrodin in rats. |
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