Effect Of Aerobic Exercise On Media Prefrontal Cortex HPPCn MRNA In Cerebral Ischemic Mice

Objective: Hepatopoietin Cn (HPPCn) is a member of the leucine-rich acidic nuclearprotein family (LANP).The expression of HPPCn was much higher in the brain thanother organs. Previous studies demonstrated that levels of HPPCn mRNA increasedfollowing tissue injury including brain stroke. But whether the stroke induced HPPCnincrease will be influenced by beneficial treatment such as pre-exercise has not beeninvestigated. This study aims to investigate the change of media Prefrontal CortexHPPCn in the process of stroke recovery improved by physical activity.Method:20six-week-old male C57BL/6mice were randomly assigned into4groups:running+ischemia group (RI group), running group(R group), ischemia group (I group)and control group (C group). RI group was treated with treadmill running for2weeksand then with2-VO cerebral ischemia for60min; R group was treated withpre-exercise and then with2-VO shame operation; I group was treated with the2-VOcerebral ischemia without pre-exercise. C group was treated with non-exercise for2weeks and then with2-VO shame operation. Realtime-PCR was used to evaluate theexpression of media Prefrontal Cortex HPPCn after the ischemia.Results:1.2-week exercise training had no significant effects on body weight and mediaPrefrontal Cortex weight.2. Compared with C group, expression of mPFC HPPCn increased significantly inR group (P＜0.05)，but expression of mPFC HPPCn didn’t change significantly in Iand RI group(P＞0.05).3. Compared with R group，expression of mPFC HPPCn decreased significantly inI and RI group (P＜0.05)Conclusion:1. Pre-exercise training can promote the expression of mPFC HPPCn significiantly.2. Ischemia after pre-exercise decreased expression of mPFC HPPCn.