| Biophoton emission is a common life phenomenon and exists in many varioussystems such as animals, plants, algae and microbial systems, it has an extreme weakintensity (typical value100photon//s·cm2) and continuous spectrum, its spectralregion is200-800nm. Bio-photon emission derives from the transition from the highenergy state to a lower state of biological molecules; it has a high degree of sensitivityto changes within biological systems and impacts of the environment. There is anintrinsic link between biophoton and the oxidative metabolism of organisms,information transfer, photosynthesis, cell division, cancer, mortality and growthregulation, and other basic life process; it has become an important research directionof the biophotonics. The bio-photon emission involves different disciplines and fields,particularly in molecular biology, biochemistry, quantum optics, non-equilibriumstatistical physics, information theory, photoelectric detection theory. For decades,scientists coming from various countries have done a lot of theoretical andexperimental study, although its mechanism has much controversy, but the bio-photonradiation provides a wealth of biological information of living systems, it has beenwidely used in the food, medicine, pharmacy, agriculture, environmental protectionand other fields.The detection and analysis of biophoton can be able to obtain microscopicinformation of the tested samples, and monitor the weak changes of the environment;it has the characteristic of fast, simple, nondestructive, so it has broad prospects for development and application, and social and economic benefits are also immeasurable.For the biophoton emission, regardless of its light-emitting mechanism theoreticalresearch and practical detection application, it, to a large extent, depends on theprogress of the detection equipment; there are not yet manufacturers to produce suchdetection equipment at home. Now we use existing advanced optical components,independently designed and built a high-performance, high-sensitivity biologicaldelayed luminescence detection system and a set of human spontaneous photonemission detection system, and are used to carry out the test and experimental studies.The main work of this paper:1. Independent design and build a high-performance, high sensitivity biophotondelayed luminescence detection system. The detection system consists of an excitationlight source, sample chamber, photomultiplier tube, photomultiplier tube coolingsystem, multi-channel photon counter, a digital delay generator, and data processing.The system is capable of measuring the delayed luminescence decay curve of manybiological samples including fresh plants, medicines, powders and other samples thedetection sensitivity of10-17W/cm2.2. Independent design and build a set of high sensitivity human spontaneous photonemission detection system, the system including the PMT, photomultiplier coolingsystem, multi-dimensional electronic control and precision positioning system.Multidimensional electronic control and precision positioning system is composed ofthe displacement frame, drive motor and sports console, the smallest movement stepof the guide can be controlled within1mm, the motion controller can achieve X, Y, Z,T1, T2axis movement of the optical detection probe. Photomultiplier tube canachieve a360°rotation, but also for around swing, the smallest mobile diagonal stepup to1°.The system can be able to achieve the precise positioning of different partsof the body, thereby measure the photon emission of different parts.3. Different biological samples have an apparent different delayed luminescence curve; our preliminary experimental studies demonstrate that the differences betweendifferent biological samples can be reflected in the level of photon emission.4. Measure the spontaneous photon emission of the palm, back, and fingers ofdifferent age’s people, we find that the photon emission: dorsum of the hand is higherthan the palm, left fingers is slightly higher than the corresponding right fingers. |
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