Comprehensive Proteomics Analysis Of Circadian Clock In Mouse Liver

An intrinsic clock enables organism to anticipate environmental changes throughaligning the physiological behavior and biochemical processes with the day/nightcycle. Specific transcription factors, which are driven by the core interlocking loopsof mammalian circadian clock, maintain the global rhythms of gene expression.Growing evidences suggest that clock is closely related to metabolic homeostasis,while the disruption of clock leads to variable diseases.Currently，a variety of high-throughput technologies were involved in the clockrelated research, such as transcriptome, epigenome and metabolome. And most ofthem are unable to interpret the mechanism of circadian clock but only describe theresults.An in-depth analysis of proteomics expression profiling is applied to describe theproteome-wide changes in mouse liver of circadian model. Together with thetranscription factors enrichment technology of catTFRE to quantitatively measure thebinding activity of TFs in mouse liver. The results demonstrate that the generaltranscriptional machinery show obvious daily behavior rhythm. And the globalrhythms of gene expression are coordinated and controlled by both the core circadiantranscriptional network and the general transcriptional machinery, then to maintain thephysiological and behavioral rhythmicity of life.Advances in this field will benefit to the development of chronotherapy tosustain human health and aging. Although we are fixed in time by the clock in genes,it s believed that drugs may be in hand to selectively reset the clock even withinspecific tissues and cells as a means of improving the adaptability to maintain healthin the near future.