Molecular Mapping Of A Golden Hull And Internode Gene In Oryza Sativa L.

The composition and metabolism of plant pigment play an important role onthe growth and development of plant. As a classical form mark character, thephenotype of gold hull and internode of rice has long been widely applied inbreeding and genetics research. This study uses Zhejing88GH which has thephenotype of gold hull and internode and was found after the being induced by60Coγ-ray irradiation for genetic analysis and molecular orientation. The results weobtained as follows:1.Compared with the wild type, the phenotype of the mutant’shull and internode is in different depth gold color.2.As using m (a)ule reagent to the lignin components analysis, Compared withwild type, Color reactions reflect that syringyl lignin’s content increased and guaiacyllignin’s content decreased in the cortex and the vascular bundle of the mutant type.3.The plant height, spikelet number, spike length,1000-grain weight and otheragronomic traits between mutant type and wild type have no significant drop.4.Genetic analysis demonstrated that the phenotype of gold hull and internodedeficiency was caused by one single recessive mutation gene in nuclear (gh6). gh6located on the short arm of chromosome2encoded a CAD protein. Sequencingresults show that, compared with LOC_Os02g09490, a single base,191th in the firstexon, mutating the first exon, mutating from C for G, from C for G, resulting inchanging serine into aianine in protein sequences coding region.and96th upstreamthe first exon, mutating from T for G.5.Biological homology analysis among each species showed that, GH6has highevolutionary relationship with the protein of sorghum and zea. I also found that GH6can translate three kinds of mRNA, they were named GHcDNA1, GHcDNA2andGHcDNA3whose cDNA numbers are AK071794.1, AK105011.2and AK059804.1.6.The mRNA expression of analysis showed LOC_Os02g09490was a constitutivegene, existed the highest expression in root and internode, than anther and palea,the expression in leaf was the lowest.7.We constructed the genome complementation vector. And did a study on the subcellular localization analysis of the protein encoded by the gene, as a result,wefound this protein located on the membrane and nucleus.This study found a new mutation of a rice gene LOC_Os02g09490, in order toprovide a theoretical basis for the further gene fu nction study and application.